A selective medium (GAMA) for the isolation of somatic cell hybrids from HPRT-AND APRT-mutant cells

Robert (Mike) Liskay, D. Patterson

Research output: Contribution to journalArticle

Abstract

A medium is described which can be used for the selection of somatic cell hybrids between HPRT-and APRT mutant cells. This medium, called GAMA, contains as its relevant constituents the following: The purines guanine and adenine and the purine biosynthetic pathway inhibitors mycophenolic adic, which blocks conversion of adenine ribonucleotides to guanine ribonucleotides, and azaserine, which blocks de novo purine synthesis.

Original languageEnglish (US)
Pages (from-to)61-69
Number of pages9
JournalCytogenetic and Genome Research
Volume23
Issue number1-2
DOIs
StatePublished - 1979
Externally publishedYes

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Ribonucleotides
Hypoxanthine Phosphoribosyltransferase
Hybrid Cells
Guanine
Adenine
Azaserine
Purines
Biosynthetic Pathways
purine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

A selective medium (GAMA) for the isolation of somatic cell hybrids from HPRT-AND APRT-mutant cells. / Liskay, Robert (Mike); Patterson, D.

In: Cytogenetic and Genome Research, Vol. 23, No. 1-2, 1979, p. 61-69.

Research output: Contribution to journalArticle

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