A selective medium (GAMA) for the isolation of somatic cell hybrids from HPRT-AND APRT-mutant cells

R. Michael Liskay; D. Patterson

doi:10.1159/000131303

A selective medium (GAMA) for the isolation of somatic cell hybrids from HPRT-AND APRT-mutant cells

R. Michael Liskay, D. Patterson

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A medium is described which can be used for the selection of somatic cell hybrids between HPRT-and APRT mutant cells. This medium, called GAMA, contains as its relevant constituents the following: The purines guanine and adenine and the purine biosynthetic pathway inhibitors mycophenolic adic, which blocks conversion of adenine ribonucleotides to guanine ribonucleotides, and azaserine, which blocks de novo purine synthesis.

Original language	English (US)
Pages (from-to)	61-69
Number of pages	9
Journal	Cytogenetics and Cell Genetics
Volume	23
Issue number	1-2
DOIs	https://doi.org/10.1159/000131303
State	Published - 1979
Externally published	Yes

ASJC Scopus subject areas

Genetics
Cell Biology

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title = "A selective medium (GAMA) for the isolation of somatic cell hybrids from HPRT-AND APRT-mutant cells",

abstract = "A medium is described which can be used for the selection of somatic cell hybrids between HPRT-and APRT mutant cells. This medium, called GAMA, contains as its relevant constituents the following: The purines guanine and adenine and the purine biosynthetic pathway inhibitors mycophenolic adic, which blocks conversion of adenine ribonucleotides to guanine ribonucleotides, and azaserine, which blocks de novo purine synthesis.",

author = "{Michael Liskay}, R. and D. Patterson",

year = "1979",

doi = "10.1159/000131303",

language = "English (US)",

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AU - Michael Liskay, R.

AU - Patterson, D.

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AB - A medium is described which can be used for the selection of somatic cell hybrids between HPRT-and APRT mutant cells. This medium, called GAMA, contains as its relevant constituents the following: The purines guanine and adenine and the purine biosynthetic pathway inhibitors mycophenolic adic, which blocks conversion of adenine ribonucleotides to guanine ribonucleotides, and azaserine, which blocks de novo purine synthesis.

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A selective medium (GAMA) for the isolation of somatic cell hybrids from HPRT-AND APRT-mutant cells

Abstract

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