A selective activity-based probe for the papain family cysteine protease dipeptidyl peptidase I/cathepsin C

Fang Yuan, Steven H L Verhelst, Galia Blum, Lisa Coussens, Matthew Bogyo

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Dipeptidyl peptidase I is involved in the activation of a number of disease-related proteases by removal of N-terminal prodipeptides. We here report a selective activity-based probe for monitoring dipeptidyl peptidase I activity in whole proteomes as well as in intact cells, without labeling of closely related enzyme family members.

Original languageEnglish (US)
Pages (from-to)5616-5617
Number of pages2
JournalJournal of the American Chemical Society
Volume128
Issue number17
DOIs
StatePublished - May 3 2006
Externally publishedYes

Fingerprint

Cathepsin C
Papain
Cysteine Proteases
Proteome
Labeling
Peptide Hydrolases
Chemical activation
Monitoring
Enzymes
Proteins
Cathepsins

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

A selective activity-based probe for the papain family cysteine protease dipeptidyl peptidase I/cathepsin C. / Yuan, Fang; Verhelst, Steven H L; Blum, Galia; Coussens, Lisa; Bogyo, Matthew.

In: Journal of the American Chemical Society, Vol. 128, No. 17, 03.05.2006, p. 5616-5617.

Research output: Contribution to journalArticle

Yuan, Fang ; Verhelst, Steven H L ; Blum, Galia ; Coussens, Lisa ; Bogyo, Matthew. / A selective activity-based probe for the papain family cysteine protease dipeptidyl peptidase I/cathepsin C. In: Journal of the American Chemical Society. 2006 ; Vol. 128, No. 17. pp. 5616-5617.
@article{eb5a7e6463c34556a940f361f0fb2e03,
title = "A selective activity-based probe for the papain family cysteine protease dipeptidyl peptidase I/cathepsin C",
abstract = "Dipeptidyl peptidase I is involved in the activation of a number of disease-related proteases by removal of N-terminal prodipeptides. We here report a selective activity-based probe for monitoring dipeptidyl peptidase I activity in whole proteomes as well as in intact cells, without labeling of closely related enzyme family members.",
author = "Fang Yuan and Verhelst, {Steven H L} and Galia Blum and Lisa Coussens and Matthew Bogyo",
year = "2006",
month = "5",
day = "3",
doi = "10.1021/ja060835v",
language = "English (US)",
volume = "128",
pages = "5616--5617",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "17",

}

TY - JOUR

T1 - A selective activity-based probe for the papain family cysteine protease dipeptidyl peptidase I/cathepsin C

AU - Yuan, Fang

AU - Verhelst, Steven H L

AU - Blum, Galia

AU - Coussens, Lisa

AU - Bogyo, Matthew

PY - 2006/5/3

Y1 - 2006/5/3

N2 - Dipeptidyl peptidase I is involved in the activation of a number of disease-related proteases by removal of N-terminal prodipeptides. We here report a selective activity-based probe for monitoring dipeptidyl peptidase I activity in whole proteomes as well as in intact cells, without labeling of closely related enzyme family members.

AB - Dipeptidyl peptidase I is involved in the activation of a number of disease-related proteases by removal of N-terminal prodipeptides. We here report a selective activity-based probe for monitoring dipeptidyl peptidase I activity in whole proteomes as well as in intact cells, without labeling of closely related enzyme family members.

UR - http://www.scopus.com/inward/record.url?scp=33646594411&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646594411&partnerID=8YFLogxK

U2 - 10.1021/ja060835v

DO - 10.1021/ja060835v

M3 - Article

C2 - 16637611

AN - SCOPUS:33646594411

VL - 128

SP - 5616

EP - 5617

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 17

ER -