A second form of gonadotropin-releasing hormone (GnRH) with characteristics of chicken GnRH-II is present in the primate brain

David W. Lescheid, Ei Terasawa, Laurie A. Abler, Henryk Urbanski, Carol M. Warby, Robert P. Millar, Nancy M. Sherwood

    Research output: Contribution to journalArticle

    148 Citations (Scopus)

    Abstract

    The primate brain was thought to contain only the GnRH known as mammalian GnRH (mGnRH). This study investigates whether a second form of GnRH exists within the primate brain. We found that brain extracts from adult stumptail and rhesus monkeys contained two forms of GnRH that were similar to mGnRH and chicken GnRH-II (cGnRH-II) based on the elution position of the peptides from HPLC and on cross-reactivity with antisera that are specific to mammalian or chicken GnRH-II in RIAs. The fetal brain of rhesus monkeys also contained mGnRH and a cGnRH-II-like peptide by the same criteria. Immunocytochemistry with a cGnRH-II-specific antiserum in adult and fetal rhesus monkeys showed immunopositive neurons generally scattered in the periaqueductal region of the midbrain, with a few positive cells in the posterior basal hypothalamus. Neurons immunopositive for cGnRH-II were fewer in number and smaller in size, with less defined nuclei and thinner neurites compared with those for mGnRH. Administration of synthetic cGnRH-II to adult rhesus monkeys resulted in a significant increase in the plasma LH concentration during the luteal phase of the menstrual cycle, but not during the midfollicular phase. We conclude that the primate brain contains mGnRH and a cGnRH-II-like molecule, although the function of the latter is unknown.

    Original languageEnglish (US)
    Pages (from-to)5618-5629
    Number of pages12
    JournalEndocrinology
    Volume138
    Issue number12
    StatePublished - 1997

    Fingerprint

    Gonadotropin-Releasing Hormone
    Primates
    Chickens
    Brain
    Macaca mulatta
    Immune Sera
    Posterior Hypothalamus
    Neurons
    Peptides
    Luteal Phase
    Neurites
    Mesencephalon
    Immunohistochemistry
    High Pressure Liquid Chromatography
    His(5)-Trp(7)-Tyr(8)-lHRH

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Lescheid, D. W., Terasawa, E., Abler, L. A., Urbanski, H., Warby, C. M., Millar, R. P., & Sherwood, N. M. (1997). A second form of gonadotropin-releasing hormone (GnRH) with characteristics of chicken GnRH-II is present in the primate brain. Endocrinology, 138(12), 5618-5629.

    A second form of gonadotropin-releasing hormone (GnRH) with characteristics of chicken GnRH-II is present in the primate brain. / Lescheid, David W.; Terasawa, Ei; Abler, Laurie A.; Urbanski, Henryk; Warby, Carol M.; Millar, Robert P.; Sherwood, Nancy M.

    In: Endocrinology, Vol. 138, No. 12, 1997, p. 5618-5629.

    Research output: Contribution to journalArticle

    Lescheid, DW, Terasawa, E, Abler, LA, Urbanski, H, Warby, CM, Millar, RP & Sherwood, NM 1997, 'A second form of gonadotropin-releasing hormone (GnRH) with characteristics of chicken GnRH-II is present in the primate brain', Endocrinology, vol. 138, no. 12, pp. 5618-5629.
    Lescheid, David W. ; Terasawa, Ei ; Abler, Laurie A. ; Urbanski, Henryk ; Warby, Carol M. ; Millar, Robert P. ; Sherwood, Nancy M. / A second form of gonadotropin-releasing hormone (GnRH) with characteristics of chicken GnRH-II is present in the primate brain. In: Endocrinology. 1997 ; Vol. 138, No. 12. pp. 5618-5629.
    @article{13e056ec1fd34f3d83cef4bba8b983af,
    title = "A second form of gonadotropin-releasing hormone (GnRH) with characteristics of chicken GnRH-II is present in the primate brain",
    abstract = "The primate brain was thought to contain only the GnRH known as mammalian GnRH (mGnRH). This study investigates whether a second form of GnRH exists within the primate brain. We found that brain extracts from adult stumptail and rhesus monkeys contained two forms of GnRH that were similar to mGnRH and chicken GnRH-II (cGnRH-II) based on the elution position of the peptides from HPLC and on cross-reactivity with antisera that are specific to mammalian or chicken GnRH-II in RIAs. The fetal brain of rhesus monkeys also contained mGnRH and a cGnRH-II-like peptide by the same criteria. Immunocytochemistry with a cGnRH-II-specific antiserum in adult and fetal rhesus monkeys showed immunopositive neurons generally scattered in the periaqueductal region of the midbrain, with a few positive cells in the posterior basal hypothalamus. Neurons immunopositive for cGnRH-II were fewer in number and smaller in size, with less defined nuclei and thinner neurites compared with those for mGnRH. Administration of synthetic cGnRH-II to adult rhesus monkeys resulted in a significant increase in the plasma LH concentration during the luteal phase of the menstrual cycle, but not during the midfollicular phase. We conclude that the primate brain contains mGnRH and a cGnRH-II-like molecule, although the function of the latter is unknown.",
    author = "Lescheid, {David W.} and Ei Terasawa and Abler, {Laurie A.} and Henryk Urbanski and Warby, {Carol M.} and Millar, {Robert P.} and Sherwood, {Nancy M.}",
    year = "1997",
    language = "English (US)",
    volume = "138",
    pages = "5618--5629",
    journal = "Endocrinology",
    issn = "0013-7227",
    publisher = "The Endocrine Society",
    number = "12",

    }

    TY - JOUR

    T1 - A second form of gonadotropin-releasing hormone (GnRH) with characteristics of chicken GnRH-II is present in the primate brain

    AU - Lescheid, David W.

    AU - Terasawa, Ei

    AU - Abler, Laurie A.

    AU - Urbanski, Henryk

    AU - Warby, Carol M.

    AU - Millar, Robert P.

    AU - Sherwood, Nancy M.

    PY - 1997

    Y1 - 1997

    N2 - The primate brain was thought to contain only the GnRH known as mammalian GnRH (mGnRH). This study investigates whether a second form of GnRH exists within the primate brain. We found that brain extracts from adult stumptail and rhesus monkeys contained two forms of GnRH that were similar to mGnRH and chicken GnRH-II (cGnRH-II) based on the elution position of the peptides from HPLC and on cross-reactivity with antisera that are specific to mammalian or chicken GnRH-II in RIAs. The fetal brain of rhesus monkeys also contained mGnRH and a cGnRH-II-like peptide by the same criteria. Immunocytochemistry with a cGnRH-II-specific antiserum in adult and fetal rhesus monkeys showed immunopositive neurons generally scattered in the periaqueductal region of the midbrain, with a few positive cells in the posterior basal hypothalamus. Neurons immunopositive for cGnRH-II were fewer in number and smaller in size, with less defined nuclei and thinner neurites compared with those for mGnRH. Administration of synthetic cGnRH-II to adult rhesus monkeys resulted in a significant increase in the plasma LH concentration during the luteal phase of the menstrual cycle, but not during the midfollicular phase. We conclude that the primate brain contains mGnRH and a cGnRH-II-like molecule, although the function of the latter is unknown.

    AB - The primate brain was thought to contain only the GnRH known as mammalian GnRH (mGnRH). This study investigates whether a second form of GnRH exists within the primate brain. We found that brain extracts from adult stumptail and rhesus monkeys contained two forms of GnRH that were similar to mGnRH and chicken GnRH-II (cGnRH-II) based on the elution position of the peptides from HPLC and on cross-reactivity with antisera that are specific to mammalian or chicken GnRH-II in RIAs. The fetal brain of rhesus monkeys also contained mGnRH and a cGnRH-II-like peptide by the same criteria. Immunocytochemistry with a cGnRH-II-specific antiserum in adult and fetal rhesus monkeys showed immunopositive neurons generally scattered in the periaqueductal region of the midbrain, with a few positive cells in the posterior basal hypothalamus. Neurons immunopositive for cGnRH-II were fewer in number and smaller in size, with less defined nuclei and thinner neurites compared with those for mGnRH. Administration of synthetic cGnRH-II to adult rhesus monkeys resulted in a significant increase in the plasma LH concentration during the luteal phase of the menstrual cycle, but not during the midfollicular phase. We conclude that the primate brain contains mGnRH and a cGnRH-II-like molecule, although the function of the latter is unknown.

    UR - http://www.scopus.com/inward/record.url?scp=0030723704&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0030723704&partnerID=8YFLogxK

    M3 - Article

    C2 - 9389550

    AN - SCOPUS:0030723704

    VL - 138

    SP - 5618

    EP - 5629

    JO - Endocrinology

    JF - Endocrinology

    SN - 0013-7227

    IS - 12

    ER -