A role for two hairpin structures as a core RNA encapsidation signal in murine leukemia virus virions

Marylene Mougel, Eric Barklis

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Four putative hairpin structures (hairpins A to D) are involved in the specific encapsidation of Moloney murine leukemia virus (M-MuLV) RNA into M- MuLV virus particles. The C and D elements, encompassing M-MuLV viral nucleotides 310 to 374, facilitate encapsidation of heterologous RNA into virions. Thus, these two elements appear to act as a core RNA encapsidation signal. The loop sequences of the putative C and D hairpins are identical (GACG). However, when GACG loops were introduced into RNAs on heterologous stem sequences, they increased encapsidation levels only three- to fourfold. These results suggest that C and D stem-and-loop sequences contribute to the M-MuLV cis-acting site for encapsidation.

Original languageEnglish (US)
Pages (from-to)8061-8065
Number of pages5
JournalJournal of Virology
Volume71
Issue number10
StatePublished - 1997

Fingerprint

Murine leukemia virus
Murine Leukemia Viruses
Moloney murine leukemia virus
virion
Virion
RNA
Inverted Repeat Sequences
stems
Nucleotides
nucleotides

ASJC Scopus subject areas

  • Immunology

Cite this

A role for two hairpin structures as a core RNA encapsidation signal in murine leukemia virus virions. / Mougel, Marylene; Barklis, Eric.

In: Journal of Virology, Vol. 71, No. 10, 1997, p. 8061-8065.

Research output: Contribution to journalArticle

@article{193aee8099414fb8854f8deb671049aa,
title = "A role for two hairpin structures as a core RNA encapsidation signal in murine leukemia virus virions",
abstract = "Four putative hairpin structures (hairpins A to D) are involved in the specific encapsidation of Moloney murine leukemia virus (M-MuLV) RNA into M- MuLV virus particles. The C and D elements, encompassing M-MuLV viral nucleotides 310 to 374, facilitate encapsidation of heterologous RNA into virions. Thus, these two elements appear to act as a core RNA encapsidation signal. The loop sequences of the putative C and D hairpins are identical (GACG). However, when GACG loops were introduced into RNAs on heterologous stem sequences, they increased encapsidation levels only three- to fourfold. These results suggest that C and D stem-and-loop sequences contribute to the M-MuLV cis-acting site for encapsidation.",
author = "Marylene Mougel and Eric Barklis",
year = "1997",
language = "English (US)",
volume = "71",
pages = "8061--8065",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "10",

}

TY - JOUR

T1 - A role for two hairpin structures as a core RNA encapsidation signal in murine leukemia virus virions

AU - Mougel, Marylene

AU - Barklis, Eric

PY - 1997

Y1 - 1997

N2 - Four putative hairpin structures (hairpins A to D) are involved in the specific encapsidation of Moloney murine leukemia virus (M-MuLV) RNA into M- MuLV virus particles. The C and D elements, encompassing M-MuLV viral nucleotides 310 to 374, facilitate encapsidation of heterologous RNA into virions. Thus, these two elements appear to act as a core RNA encapsidation signal. The loop sequences of the putative C and D hairpins are identical (GACG). However, when GACG loops were introduced into RNAs on heterologous stem sequences, they increased encapsidation levels only three- to fourfold. These results suggest that C and D stem-and-loop sequences contribute to the M-MuLV cis-acting site for encapsidation.

AB - Four putative hairpin structures (hairpins A to D) are involved in the specific encapsidation of Moloney murine leukemia virus (M-MuLV) RNA into M- MuLV virus particles. The C and D elements, encompassing M-MuLV viral nucleotides 310 to 374, facilitate encapsidation of heterologous RNA into virions. Thus, these two elements appear to act as a core RNA encapsidation signal. The loop sequences of the putative C and D hairpins are identical (GACG). However, when GACG loops were introduced into RNAs on heterologous stem sequences, they increased encapsidation levels only three- to fourfold. These results suggest that C and D stem-and-loop sequences contribute to the M-MuLV cis-acting site for encapsidation.

UR - http://www.scopus.com/inward/record.url?scp=0030756546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030756546&partnerID=8YFLogxK

M3 - Article

VL - 71

SP - 8061

EP - 8065

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 10

ER -