A role for two hairpin structures as a core RNA encapsidation signal in murine leukemia virus virions

Marylene Mougel, Eric Barklis

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48 Scopus citations

Abstract

Four putative hairpin structures (hairpins A to D) are involved in the specific encapsidation of Moloney murine leukemia virus (M-MuLV) RNA into M- MuLV virus particles. The C and D elements, encompassing M-MuLV viral nucleotides 310 to 374, facilitate encapsidation of heterologous RNA into virions. Thus, these two elements appear to act as a core RNA encapsidation signal. The loop sequences of the putative C and D hairpins are identical (GACG). However, when GACG loops were introduced into RNAs on heterologous stem sequences, they increased encapsidation levels only three- to fourfold. These results suggest that C and D stem-and-loop sequences contribute to the M-MuLV cis-acting site for encapsidation.

Original languageEnglish (US)
Pages (from-to)8061-8065
Number of pages5
JournalJournal of virology
Volume71
Issue number10
StatePublished - Sep 26 1997

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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