A role for fasciclin II in the guidance of neuronal migration

Jay W. Wright, Mark A. Snyder, Kristine M. Schwinof, Stacey Combes, Philip Copenhaver

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The insect cell adhesion receptor fasciclin II is expressed by specific subsets of neural and non-neural cells during embryogenesis and has been shown to control growth cone motility and axonal fasciculation. Here we demonstrate a role for fasciclin II in the guidance of migratory neurons. In the developing enteric nervous system of the moth Manduca sexta, an identified set of neurons (the EP cells) undergoes a stereotyped sequence of migration along the visceral muscle bands of the midgut prior to their differentiation. Probes specific for Manduca fasciclin II show that while the EP cells express fasciclin II throughout embryogenesis, their muscle band pathways express fasciclin II only during the migratory period. Manipulations of fasciclin II in embryonic culture using blocking antibodies, recombinant fasciclin II fragments, and enzymatic removal of glycosyl phosphatidylinositol-linked fasciclin II produced concentration-dependent reductions in the extent of EP cell migration. These results support a novel role for fasciclin II, indicating that this homophilic adhesion molecule is required for the promotion or guidance of neuronal migration.

Original languageEnglish (US)
Pages (from-to)3217-3228
Number of pages12
JournalDevelopment
Volume126
Issue number14
StatePublished - Jul 1999

Fingerprint

Manduca
Embryonic Development
Enteric Nervous System
Axon Guidance
fasciclin II
Neurons
Muscles
Glycosylphosphatidylinositols
Growth Cones
Blocking Antibodies
Moths
Cell Adhesion
Cell Movement
Insects
Axon Fasciculation
adhesion receptor

Keywords

  • Adhesion molecule
  • Fasciclin II
  • Guidance
  • Manduca sexta
  • Migration

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Wright, J. W., Snyder, M. A., Schwinof, K. M., Combes, S., & Copenhaver, P. (1999). A role for fasciclin II in the guidance of neuronal migration. Development, 126(14), 3217-3228.

A role for fasciclin II in the guidance of neuronal migration. / Wright, Jay W.; Snyder, Mark A.; Schwinof, Kristine M.; Combes, Stacey; Copenhaver, Philip.

In: Development, Vol. 126, No. 14, 07.1999, p. 3217-3228.

Research output: Contribution to journalArticle

Wright, JW, Snyder, MA, Schwinof, KM, Combes, S & Copenhaver, P 1999, 'A role for fasciclin II in the guidance of neuronal migration', Development, vol. 126, no. 14, pp. 3217-3228.
Wright JW, Snyder MA, Schwinof KM, Combes S, Copenhaver P. A role for fasciclin II in the guidance of neuronal migration. Development. 1999 Jul;126(14):3217-3228.
Wright, Jay W. ; Snyder, Mark A. ; Schwinof, Kristine M. ; Combes, Stacey ; Copenhaver, Philip. / A role for fasciclin II in the guidance of neuronal migration. In: Development. 1999 ; Vol. 126, No. 14. pp. 3217-3228.
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