A ribose modification of Spinach aptamer accelerates lead(ii) cation association

In vitro

Jonathan C. Savage, Pushkar Shinde, Hans Peter Bächinger, Monika Davare, Ujwal Shinde

Research output: Contribution to journalArticle

Abstract

Spinach aptamer fluorescence requires formation of a tripartite complex composed of folded RNA, a GFP-like fluorophore, and selective cation coordination. 2′F pyrimidine modified Spinach has retained fluorescence, increased chemical stability, and accelerated cation association via increased G-quadruplex dynamics, thereby reducing readout time and enhancing Spinach utility for aqueous Pb 2+ detection.

Original languageEnglish (US)
Pages (from-to)5882-5885
Number of pages4
JournalChemical Communications
Volume55
Issue number42
DOIs
StatePublished - Jan 1 2019

Fingerprint

Ribose
Cations
Lead
Positive ions
Fluorescence
Association reactions
Fluorophores
Chemical stability
RNA
pyrimidine

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

A ribose modification of Spinach aptamer accelerates lead(ii) cation association : In vitro. / Savage, Jonathan C.; Shinde, Pushkar; Bächinger, Hans Peter; Davare, Monika; Shinde, Ujwal.

In: Chemical Communications, Vol. 55, No. 42, 01.01.2019, p. 5882-5885.

Research output: Contribution to journalArticle

Savage, Jonathan C. ; Shinde, Pushkar ; Bächinger, Hans Peter ; Davare, Monika ; Shinde, Ujwal. / A ribose modification of Spinach aptamer accelerates lead(ii) cation association : In vitro. In: Chemical Communications. 2019 ; Vol. 55, No. 42. pp. 5882-5885.
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