A real-time view of life within 100 NM of the plasma membrane

J. A. Steyer, Wolfhard Almers

Research output: Contribution to journalArticle

313 Citations (Scopus)

Abstract

The plasma membrane is a two-dimensional compartment that relays most biological signals sent or received by a cell. Signalling involves membrane receptors and their associated enzyme cascades as well as organelles such as exocytic and endocytic vesicles. Advances in light microscope design, new organelle-specific vital stains and fluorescent proteins have renewed the interest in evanescent field fluorescence microscopy, a method uniquely suited to image the plasma membrane with its associated organelles and macromolecules in living cells. The method shows even the smallest vesicles made by cells, and can image the dynamics of single protein molecules.

Original languageEnglish (US)
Pages (from-to)268-275
Number of pages8
JournalNature Reviews Molecular Cell Biology
Volume2
Issue number4
DOIs
StatePublished - Apr 2001

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Organelles
Cell Membrane
Transport Vesicles
Fluorescence Microscopy
Proteins
Coloring Agents
Light
Membranes
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

A real-time view of life within 100 NM of the plasma membrane. / Steyer, J. A.; Almers, Wolfhard.

In: Nature Reviews Molecular Cell Biology, Vol. 2, No. 4, 04.2001, p. 268-275.

Research output: Contribution to journalArticle

Steyer, J. A. ; Almers, Wolfhard. / A real-time view of life within 100 NM of the plasma membrane. In: Nature Reviews Molecular Cell Biology. 2001 ; Vol. 2, No. 4. pp. 268-275.
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