A real-time view of life within 100 NM of the plasma membrane

J. A. Steyer, W. Almers

Research output: Contribution to journalReview articlepeer-review

336 Scopus citations


The plasma membrane is a two-dimensional compartment that relays most biological signals sent or received by a cell. Signalling involves membrane receptors and their associated enzyme cascades as well as organelles such as exocytic and endocytic vesicles. Advances in light microscope design, new organelle-specific vital stains and fluorescent proteins have renewed the interest in evanescent field fluorescence microscopy, a method uniquely suited to image the plasma membrane with its associated organelles and macromolecules in living cells. The method shows even the smallest vesicles made by cells, and can image the dynamics of single protein molecules.

Original languageEnglish (US)
Pages (from-to)268-275
Number of pages8
JournalNature Reviews Molecular Cell Biology
Issue number4
StatePublished - Apr 1 2001

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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