Abstract
The plasma membrane is a two-dimensional compartment that relays most biological signals sent or received by a cell. Signalling involves membrane receptors and their associated enzyme cascades as well as organelles such as exocytic and endocytic vesicles. Advances in light microscope design, new organelle-specific vital stains and fluorescent proteins have renewed the interest in evanescent field fluorescence microscopy, a method uniquely suited to image the plasma membrane with its associated organelles and macromolecules in living cells. The method shows even the smallest vesicles made by cells, and can image the dynamics of single protein molecules.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 268-275 |
| Number of pages | 8 |
| Journal | Nature Reviews Molecular Cell Biology |
| Volume | 2 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2001 |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
A real-time view of life within 100 NM of the plasma membrane. / Steyer, J. A.; Almers, Wolfhard.
In: Nature Reviews Molecular Cell Biology, Vol. 2, No. 4, 04.2001, p. 268-275.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A real-time view of life within 100 NM of the plasma membrane
AU - Steyer, J. A.
AU - Almers, Wolfhard
PY - 2001/4
Y1 - 2001/4
N2 - The plasma membrane is a two-dimensional compartment that relays most biological signals sent or received by a cell. Signalling involves membrane receptors and their associated enzyme cascades as well as organelles such as exocytic and endocytic vesicles. Advances in light microscope design, new organelle-specific vital stains and fluorescent proteins have renewed the interest in evanescent field fluorescence microscopy, a method uniquely suited to image the plasma membrane with its associated organelles and macromolecules in living cells. The method shows even the smallest vesicles made by cells, and can image the dynamics of single protein molecules.
AB - The plasma membrane is a two-dimensional compartment that relays most biological signals sent or received by a cell. Signalling involves membrane receptors and their associated enzyme cascades as well as organelles such as exocytic and endocytic vesicles. Advances in light microscope design, new organelle-specific vital stains and fluorescent proteins have renewed the interest in evanescent field fluorescence microscopy, a method uniquely suited to image the plasma membrane with its associated organelles and macromolecules in living cells. The method shows even the smallest vesicles made by cells, and can image the dynamics of single protein molecules.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035315984&partnerID=8YFLogxK
U2 - 10.1038/35067069
DO - 10.1038/35067069
M3 - Article
C2 - 11283724
AN - SCOPUS:0035315984
VL - 2
SP - 268
EP - 275
JO - Nature Reviews Molecular Cell Biology
JF - Nature Reviews Molecular Cell Biology
SN - 1471-0072
IS - 4
ER -