Abstract
A precipitation-filtration assay which measures the activity of nicotinamide phosphoribosyltransferase is described. The assay, based on the differential solubility of nicotinamide and nicotinamide mononucleotide in acetone, is rapid, convenient, and inexpensive when compared to chromatographic methods. It is sensitive enough to detect the formation of as little as 10 pmol of nicotinamide mononucleotide under the reaction conditions described. With minor modifications, the assay procedure can be adapted for measuring a variety of other enzymes.
Original language | English (US) |
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Pages (from-to) | 199-205 |
Number of pages | 7 |
Journal | Analytical Biochemistry |
Volume | 107 |
Issue number | 1 |
DOIs | |
State | Published - 1980 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology