A rapid procedure for assaying nicotinamide phosphoribosyltransferase

George C. Elliott, James Ajioka, Craig Y. Okada

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A precipitation-filtration assay which measures the activity of nicotinamide phosphoribosyltransferase is described. The assay, based on the differential solubility of nicotinamide and nicotinamide mononucleotide in acetone, is rapid, convenient, and inexpensive when compared to chromatographic methods. It is sensitive enough to detect the formation of as little as 10 pmol of nicotinamide mononucleotide under the reaction conditions described. With minor modifications, the assay procedure can be adapted for measuring a variety of other enzymes.

Original languageEnglish (US)
Pages (from-to)199-205
Number of pages7
JournalAnalytical Biochemistry
Volume107
Issue number1
DOIs
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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