A rapid procedure for assaying nicotinamide phosphoribosyltransferase

G. C. Elliott, J. Ajioka, Craig Okada

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A precipitation-filtration assay which measures the activity of nicotinamide phosphoribosyltransferase is described. The assay, based on the differential solubility of nicotinamide and nicotinamide mononucleotide in acetone, is rapid, convenient, and inexpensive when compared to chromatographic methods. It is sensitive enough to detect the formation of as little as 10 pmol of nicotinamide mononucleotide under the reaction conditions described. With minor modifications, the assay procedure can be adapted for measuring a variety of other enzymes.

Original languageEnglish (US)
Pages (from-to)199-205
Number of pages7
JournalAnalytical Biochemistry
Volume107
Issue number1
StatePublished - 1980
Externally publishedYes

Fingerprint

Nicotinamide Mononucleotide
Nicotinamide Phosphoribosyltransferase
Assays
Niacinamide
Acetone
Solubility
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A rapid procedure for assaying nicotinamide phosphoribosyltransferase. / Elliott, G. C.; Ajioka, J.; Okada, Craig.

In: Analytical Biochemistry, Vol. 107, No. 1, 1980, p. 199-205.

Research output: Contribution to journalArticle

Elliott, G. C. ; Ajioka, J. ; Okada, Craig. / A rapid procedure for assaying nicotinamide phosphoribosyltransferase. In: Analytical Biochemistry. 1980 ; Vol. 107, No. 1. pp. 199-205.
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