A rapid, nongenomic action of glucocorticoids in rat B103 neuroblastoma cells

Jian Zhong Han, Wen Lin, Shu Jie Lou, Jian Qiu, Yi Zhang Chen

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We report here a new example in which glucocorticoids (GCs) acted in a rapid, nongenomic way. In rat B103 neuroblastoma cells, 5-hydroxytryptamine (5-HT) was found to evoke an immediate rise in intracellular free calcium concentration ([Ca2+]i). Pre-incubation of B103 cells for 5 min with corticosterone (B) or bovine serum albumin-conjugated corticosterone (B-BSA) concentration-dependently (10-4-10-8 M) inhibited the peak increments in [Ca2+]i. Cortisol and dexamethasone had a similar effect, while deoxycorticosterone and cholesterol were ineffective. This rapid inhibitory effect of corticosterone could be mimicked by protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) and abolished completely by PKC inhibitors Ro31-8220 or GF-109203X. Neither pertussis toxin (PTX) nor nuclear GC receptor (GR) antagonist RU38486 influenced the rapid action of B. Our results suggest that GCs can modulate the 5-HT-induced Ca2+ response in B103 cells in a membrane-initiated, nongenomic, and PKC-dependent manner.

Original languageEnglish (US)
Pages (from-to)21-27
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1591
Issue number1-3
DOIs
StatePublished - Aug 19 2002

Keywords

  • 5-Hydroxytryptamine
  • Calcium
  • Glucocorticoid
  • Protein kinase C

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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