A rapid, nongenomic action of glucocorticoids in rat B103 neuroblastoma cells

Jian Zhong Han, Wen Lin, Shu Jie Lou, Jian Qiu, Yi Zhang Chen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We report here a new example in which glucocorticoids (GCs) acted in a rapid, nongenomic way. In rat B103 neuroblastoma cells, 5-hydroxytryptamine (5-HT) was found to evoke an immediate rise in intracellular free calcium concentration ([Ca2+]i). Pre-incubation of B103 cells for 5 min with corticosterone (B) or bovine serum albumin-conjugated corticosterone (B-BSA) concentration-dependently (10-4-10-8 M) inhibited the peak increments in [Ca2+]i. Cortisol and dexamethasone had a similar effect, while deoxycorticosterone and cholesterol were ineffective. This rapid inhibitory effect of corticosterone could be mimicked by protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) and abolished completely by PKC inhibitors Ro31-8220 or GF-109203X. Neither pertussis toxin (PTX) nor nuclear GC receptor (GR) antagonist RU38486 influenced the rapid action of B. Our results suggest that GCs can modulate the 5-HT-induced Ca2+ response in B103 cells in a membrane-initiated, nongenomic, and PKC-dependent manner.

Original languageEnglish (US)
Pages (from-to)21-27
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1591
Issue number1-3
DOIs
StatePublished - Aug 19 2002
Externally publishedYes

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Neuroblastoma
Protein Kinase C
Glucocorticoids
Corticosterone
Serotonin
Desoxycorticosterone
Protein C Inhibitor
Pertussis Toxin
Glucocorticoid Receptors
Protein Kinase Inhibitors
Cytoplasmic and Nuclear Receptors
Dexamethasone
Hydrocortisone
Acetates
Cholesterol
Calcium
Membranes

Keywords

  • 5-Hydroxytryptamine
  • Calcium
  • Glucocorticoid
  • Protein kinase C

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

Cite this

A rapid, nongenomic action of glucocorticoids in rat B103 neuroblastoma cells. / Han, Jian Zhong; Lin, Wen; Lou, Shu Jie; Qiu, Jian; Chen, Yi Zhang.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1591, No. 1-3, 19.08.2002, p. 21-27.

Research output: Contribution to journalArticle

Han, Jian Zhong ; Lin, Wen ; Lou, Shu Jie ; Qiu, Jian ; Chen, Yi Zhang. / A rapid, nongenomic action of glucocorticoids in rat B103 neuroblastoma cells. In: Biochimica et Biophysica Acta - Molecular Cell Research. 2002 ; Vol. 1591, No. 1-3. pp. 21-27.
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