A rapid and sensitive LC-MS/MS method for quantification of four anthocyanins and its application in a clinical pharmacology study of a bioadhesive black raspberry gel

Yonghua Ling, Chen Ren, Susan R. Mallery, Carlos Ugalde, Ping Pei, U. V R Vijaya Saradhi, Gary D. Stoner, Kenneth K. Chan, Zhongfa Liu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Cyanidin 3-glucoside (C3GLU), cyanidin 3-rutinoside (C3RUT), cyanidin 3-sambubioside (C3SAM) and cyanidin 3-(2G-xylosyl) rutinoside (C3XRUT) are the four constituent black raspberry anthocyanins that contribute significantly to the chemopreventive effects of freeze-dried black raspberries (FBR). A highly sensitive and specific LC-MS/MS assay was developed and validated to simultaneously quantify these four FBR anthocyanins in human saliva, plasma and oral tissue homogenates. In saliva, the lower limit of quantification (LLOQ) for these anthocyanins was 1.0 ng/mL. The within-run and between-run coefficients of variations (CVs) at the quality control concentrations (1.0, 5.0, 50 and 500 ng/mL) were all

Original languageEnglish (US)
Pages (from-to)4027-4034
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume877
Issue number31
DOIs
StatePublished - Dec 1 2009
Externally publishedYes

Fingerprint

Anthocyanins
Clinical Pharmacology
Gels
Saliva
Plasma (human)
Quality Control
Quality control
Assays
Tissue
Rubus
Clinical Studies

Keywords

  • Anthocyanins
  • Bioadhesive black raspberry gel
  • LC-MS/MS
  • Pharmacology
  • Quantitation

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

A rapid and sensitive LC-MS/MS method for quantification of four anthocyanins and its application in a clinical pharmacology study of a bioadhesive black raspberry gel. / Ling, Yonghua; Ren, Chen; Mallery, Susan R.; Ugalde, Carlos; Pei, Ping; Saradhi, U. V R Vijaya; Stoner, Gary D.; Chan, Kenneth K.; Liu, Zhongfa.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 31, 01.12.2009, p. 4027-4034.

Research output: Contribution to journalArticle

Ling, Yonghua ; Ren, Chen ; Mallery, Susan R. ; Ugalde, Carlos ; Pei, Ping ; Saradhi, U. V R Vijaya ; Stoner, Gary D. ; Chan, Kenneth K. ; Liu, Zhongfa. / A rapid and sensitive LC-MS/MS method for quantification of four anthocyanins and its application in a clinical pharmacology study of a bioadhesive black raspberry gel. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2009 ; Vol. 877, No. 31. pp. 4027-4034.
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abstract = "Cyanidin 3-glucoside (C3GLU), cyanidin 3-rutinoside (C3RUT), cyanidin 3-sambubioside (C3SAM) and cyanidin 3-(2G-xylosyl) rutinoside (C3XRUT) are the four constituent black raspberry anthocyanins that contribute significantly to the chemopreventive effects of freeze-dried black raspberries (FBR). A highly sensitive and specific LC-MS/MS assay was developed and validated to simultaneously quantify these four FBR anthocyanins in human saliva, plasma and oral tissue homogenates. In saliva, the lower limit of quantification (LLOQ) for these anthocyanins was 1.0 ng/mL. The within-run and between-run coefficients of variations (CVs) at the quality control concentrations (1.0, 5.0, 50 and 500 ng/mL) were all",
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