A Quantitative Assay for Telomerase Enzyme Activity Predicts Severity of Disease in Neuroblastoma

Leroy Trombetta; Tommy Brown; Kathy Moore; Mary Joe Dehart; Robert Sawin; Kenneth Azarow

doi:10.1016/S0149-7944(99)00155-5

A Quantitative Assay for Telomerase Enzyme Activity Predicts Severity of Disease in Neuroblastoma

Leroy Trombetta, Tommy Brown, Kathy Moore, Mary Joe Dehart, Robert Sawin, Kenneth Azarow

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Purpose: To determine whether telomerase enzyme activity correlates with tumor stage, grade, and prognosis using a quantitative telomerase assay. Method: A new semiquantitative assay for telomerase enzyme activity (Telomerase Repeat Amplification Protocol-enzyme-linked immunosorbent assay) was employed on 21 neuroblastoma specimens. Enzyme-linked immunosorbent assay results were compared to tumor stage, patient age, and overall tumor aggressiveness. The principle investigator was blinded to all clinical data. A Student's t test was used to determine significance (p < 0.05). Results: Telomerase activity correlated with stage of tumor (p < 0.007). Age of patient did not correlate with telomerase activity independently. Conclusions: Telomerase enzyme activity was predictive for neuroblastoma stage. This implies that increased telomerase activity is involved in tumor dedifferentiation. The semiquantitative telomerase assay may be used as a rapid prognostic indicator and serve as a guide to initiating treatment in childhood neuroblastoma.

Original language	English (US)
Pages (from-to)	413-416
Number of pages	4
Journal	Current Surgery
Volume	56
Issue number	7-8
DOIs	https://doi.org/10.1016/S0149-7944(99)00155-5
State	Published - 1999
Externally published	Yes

ASJC Scopus subject areas

Surgery

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10.1016/S0149-7944(99)00155-5

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title = "A Quantitative Assay for Telomerase Enzyme Activity Predicts Severity of Disease in Neuroblastoma",

abstract = "Purpose: To determine whether telomerase enzyme activity correlates with tumor stage, grade, and prognosis using a quantitative telomerase assay. Method: A new semiquantitative assay for telomerase enzyme activity (Telomerase Repeat Amplification Protocol-enzyme-linked immunosorbent assay) was employed on 21 neuroblastoma specimens. Enzyme-linked immunosorbent assay results were compared to tumor stage, patient age, and overall tumor aggressiveness. The principle investigator was blinded to all clinical data. A Student's t test was used to determine significance (p < 0.05). Results: Telomerase activity correlated with stage of tumor (p < 0.007). Age of patient did not correlate with telomerase activity independently. Conclusions: Telomerase enzyme activity was predictive for neuroblastoma stage. This implies that increased telomerase activity is involved in tumor dedifferentiation. The semiquantitative telomerase assay may be used as a rapid prognostic indicator and serve as a guide to initiating treatment in childhood neuroblastoma.",

author = "Leroy Trombetta and Tommy Brown and Kathy Moore and Dehart, {Mary Joe} and Robert Sawin and Kenneth Azarow",

note = "Funding Information: All financial support for this research has been provided by the Department of Clinical Investigations, Madigan Army Medical Center, United States Army. The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or reflecting the views of the Department of the Army or the Department of Defense. ",

year = "1999",

doi = "10.1016/S0149-7944(99)00155-5",

language = "English (US)",

volume = "56",

pages = "413--416",

journal = "Current Surgery",

issn = "0149-7944",

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T1 - A Quantitative Assay for Telomerase Enzyme Activity Predicts Severity of Disease in Neuroblastoma

AU - Trombetta, Leroy

AU - Brown, Tommy

AU - Moore, Kathy

AU - Dehart, Mary Joe

AU - Sawin, Robert

AU - Azarow, Kenneth

N1 - Funding Information: All financial support for this research has been provided by the Department of Clinical Investigations, Madigan Army Medical Center, United States Army. The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or reflecting the views of the Department of the Army or the Department of Defense.

PY - 1999

Y1 - 1999

N2 - Purpose: To determine whether telomerase enzyme activity correlates with tumor stage, grade, and prognosis using a quantitative telomerase assay. Method: A new semiquantitative assay for telomerase enzyme activity (Telomerase Repeat Amplification Protocol-enzyme-linked immunosorbent assay) was employed on 21 neuroblastoma specimens. Enzyme-linked immunosorbent assay results were compared to tumor stage, patient age, and overall tumor aggressiveness. The principle investigator was blinded to all clinical data. A Student's t test was used to determine significance (p < 0.05). Results: Telomerase activity correlated with stage of tumor (p < 0.007). Age of patient did not correlate with telomerase activity independently. Conclusions: Telomerase enzyme activity was predictive for neuroblastoma stage. This implies that increased telomerase activity is involved in tumor dedifferentiation. The semiquantitative telomerase assay may be used as a rapid prognostic indicator and serve as a guide to initiating treatment in childhood neuroblastoma.

AB - Purpose: To determine whether telomerase enzyme activity correlates with tumor stage, grade, and prognosis using a quantitative telomerase assay. Method: A new semiquantitative assay for telomerase enzyme activity (Telomerase Repeat Amplification Protocol-enzyme-linked immunosorbent assay) was employed on 21 neuroblastoma specimens. Enzyme-linked immunosorbent assay results were compared to tumor stage, patient age, and overall tumor aggressiveness. The principle investigator was blinded to all clinical data. A Student's t test was used to determine significance (p < 0.05). Results: Telomerase activity correlated with stage of tumor (p < 0.007). Age of patient did not correlate with telomerase activity independently. Conclusions: Telomerase enzyme activity was predictive for neuroblastoma stage. This implies that increased telomerase activity is involved in tumor dedifferentiation. The semiquantitative telomerase assay may be used as a rapid prognostic indicator and serve as a guide to initiating treatment in childhood neuroblastoma.

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EP - 416

JO - Current Surgery

JF - Current Surgery

IS - 7-8

ER -

A Quantitative Assay for Telomerase Enzyme Activity Predicts Severity of Disease in Neuroblastoma

Abstract

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