A Quantitative Assay for Telomerase Enzyme Activity Predicts Severity of Disease in Neuroblastoma

Leroy Trombetta, Tommy Brown, Kathy Moore, Mary Joe Dehart, Robert Sawin, Kenneth Azarow

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: To determine whether telomerase enzyme activity correlates with tumor stage, grade, and prognosis using a quantitative telomerase assay. Method: A new semiquantitative assay for telomerase enzyme activity (Telomerase Repeat Amplification Protocol-enzyme-linked immunosorbent assay) was employed on 21 neuroblastoma specimens. Enzyme-linked immunosorbent assay results were compared to tumor stage, patient age, and overall tumor aggressiveness. The principle investigator was blinded to all clinical data. A Student's t test was used to determine significance (p <0.05). Results: Telomerase activity correlated with stage of tumor (p <0.007). Age of patient did not correlate with telomerase activity independently. Conclusions: Telomerase enzyme activity was predictive for neuroblastoma stage. This implies that increased telomerase activity is involved in tumor dedifferentiation. The semiquantitative telomerase assay may be used as a rapid prognostic indicator and serve as a guide to initiating treatment in childhood neuroblastoma.

Original languageEnglish (US)
Pages (from-to)413-416
Number of pages4
JournalCurrent Surgery
Volume56
Issue number7-8
StatePublished - Sep 1999
Externally publishedYes

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Telomerase
Enzyme Assays
Neuroblastoma
Disease
Neoplasms
aggressiveness
Enzyme-Linked Immunosorbent Assay
childhood
Enzymes
Research Personnel
Students
student

ASJC Scopus subject areas

  • Surgery

Cite this

Trombetta, L., Brown, T., Moore, K., Dehart, M. J., Sawin, R., & Azarow, K. (1999). A Quantitative Assay for Telomerase Enzyme Activity Predicts Severity of Disease in Neuroblastoma. Current Surgery, 56(7-8), 413-416.

A Quantitative Assay for Telomerase Enzyme Activity Predicts Severity of Disease in Neuroblastoma. / Trombetta, Leroy; Brown, Tommy; Moore, Kathy; Dehart, Mary Joe; Sawin, Robert; Azarow, Kenneth.

In: Current Surgery, Vol. 56, No. 7-8, 09.1999, p. 413-416.

Research output: Contribution to journalArticle

Trombetta, L, Brown, T, Moore, K, Dehart, MJ, Sawin, R & Azarow, K 1999, 'A Quantitative Assay for Telomerase Enzyme Activity Predicts Severity of Disease in Neuroblastoma', Current Surgery, vol. 56, no. 7-8, pp. 413-416.
Trombetta, Leroy ; Brown, Tommy ; Moore, Kathy ; Dehart, Mary Joe ; Sawin, Robert ; Azarow, Kenneth. / A Quantitative Assay for Telomerase Enzyme Activity Predicts Severity of Disease in Neuroblastoma. In: Current Surgery. 1999 ; Vol. 56, No. 7-8. pp. 413-416.
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