A Putative New Growth Factor in Ascitic Fluid from Ovarian Cancer Patients: Identification, Characterization, and Mechanism of Action

Gordon B. Mills, Christopher May, Martha McGill, Chaim M. Roifman, Alan Mellors

    Research output: Contribution to journalArticle

    107 Scopus citations

    Abstract

    Ascitic fluid from ovarian cancer patients (n = 16), but not from patients with other cancers or with benign diseases, contains a growth-promoting activity which induces the proliferation of both fresh ovarian cancer cells (a = 5) and the ovarian cancer cell line HEY. The ascitic fluid growth factor(s) appears to signal cells through binding and activation of specific, saturable, high-affinity cell surface receptors. Incubation of fresh or cultured ovarian cancer cells with a partially purified preparation of ascitic fluid stimulates phosphatidylinositol turnover and increases cytosolic-free calcium. Each of these biochemical events has been implicated in the action of growth factors. Purified preparations of previously identified growth factors including epidermal growth factor, transforming growth factor-β, tumor necrosis factor, platelet-derived growth factor, thrombin, insulin, interleukin-1, interleukin-2, vasopressin, angiotensin, a-and β-interferons, and fibroblast growth factor did not increase cytosolic-free calcium in either fresh ovarian cancer cells or HEY cells. Therefore, ascitic fluid appears to contain one or more previously unidentified growth factors which activate ovarian cancer cells through phosphatidylinositol hydrolysis and resultant changes in cyto-solic-free calcium.

    Original languageEnglish (US)
    Pages (from-to)1066-1071
    Number of pages6
    JournalCancer Research
    Volume48
    Issue number5
    StatePublished - Jan 1 1988

    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

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