A programmable system to perform the polymerase chain reaction.

H. U. Weier, Joe Gray

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An automated system is described that performs the cyclic temperature changes required for enzymatic amplification of specific DNA segments in vitro using the polymerase chain reaction (pcr). During pcr, oligonucleotide primer molecules are bound at low temperature to templates of heat-denatured DNA and extended on their 3' end using a thermostable DNA polymerase. The DNA denaturation, primer annealing, and extension is repeated several times under program control to accumulate a large number of identical copies of the DNA sequence between the primers. A microcomputer system controls the flow of 96 degrees C and 37 degrees C water through a 24-well sample holder so that the temperature in the samples in the holder varies as required for DNA denaturation, primer annealing, and DNA polymerization. The microcomputer automatically performs multiple thermal cycles and is sufficiently flexible that the temperature profile can be varied from cycle to cycle.

Original languageEnglish (US)
Pages (from-to)441-447
Number of pages7
JournalDNA
Volume7
Issue number6
StatePublished - Jul 1988
Externally publishedYes

Fingerprint

Polymerase chain reaction
DNA Primers
Nucleic Acid Denaturation
Polymerase Chain Reaction
Denaturation
Temperature
Microcomputers
DNA
Hot Temperature
Annealing
DNA sequences
DNA-Directed DNA Polymerase
Polymerization
Amplification
Control systems
Molecules
Water

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

A programmable system to perform the polymerase chain reaction. / Weier, H. U.; Gray, Joe.

In: DNA, Vol. 7, No. 6, 07.1988, p. 441-447.

Research output: Contribution to journalArticle

Weier, H. U. ; Gray, Joe. / A programmable system to perform the polymerase chain reaction. In: DNA. 1988 ; Vol. 7, No. 6. pp. 441-447.
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