A practical method for rescuing desired hybridomas during monoclonal antibody production

Grazyna Adamus, Z. Suzanne Zam, Scotts Emerson, Paul A. Hargrave

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We present a practical method for the rescue of previosly stable hybridoma clones which increases the proportion of desired cells in the population before cloning by limiting dilution. When the antibody activity of a culture supernatant was lower than that previously obtained, a precloning distribution at a density of 10 cells per microtiter well greatly improved the chances of obtaining a single active clone by subsequent limiting dilution. The Poisson distribution model was used to evaluate the method. Probabilities calculated clearly demonstrate the advantage of this precloning distribution step when attempting to isolate a hydridoma cell line that is relatively rare in a population.

Original languageEnglish (US)
Pages (from-to)1141-1146
Number of pages6
JournalIn Vitro Cellular & Developmental Biology
Volume25
Issue number12
DOIs
StatePublished - Dec 1 1989

Keywords

  • hybridoma
  • limiting dilution cloning
  • monoclonal antibody

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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