A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in the arcuate nucleus

Edward J. Wagner, Martha A. Bosch, Martin J. Kelly, K. Rønnekleiv

Research output: Contribution to journalArticle

6 Scopus citations


We combined histofluorescence with in situ hybridization to identify GABAergic neurons in the arcuate nucleus (ARC) following electrophysiological recordings, using GAD65 as a marker. Intracellular recordings were made in hypothalamic slices prepared from ovariectomized guinea pigs. Over 90% of ARC neurons tested with the GABA(B) receptor agonist baclofen responded with a membrane hyperpolarization or an outward current. The hyperpolarization was dose-dependent, and the GABA(B) receptor antagonist CGP 35,348 produced a rightward shift in the agonist dose-response curve. Agonist potency was lower, and the efficacy greater, in GAD-positive neurons. The use of this novel technique for identifying GABAergic neurons thus reveals differences in the pharmacodynamics of GABA(B) receptor activation GABAergic and non- GABAergic ARC neurons.

Original languageEnglish (US)
Pages (from-to)4171-4177
Number of pages7
Issue number18
StatePublished - Dec 21 1998



  • Arcuate nucleus
  • Electrophysiology
  • GABA
  • Glutamatic acid decarboxylase
  • In situ hybridization
  • Potassium conductance

ASJC Scopus subject areas

  • Neuroscience(all)

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