A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in the arcuate nucleus

Edward J. Wagner, Martha A. Bosch, Martin Kelly, K. Rønnekleiv

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We combined histofluorescence with in situ hybridization to identify GABAergic neurons in the arcuate nucleus (ARC) following electrophysiological recordings, using GAD65 as a marker. Intracellular recordings were made in hypothalamic slices prepared from ovariectomized guinea pigs. Over 90% of ARC neurons tested with the GABA(B) receptor agonist baclofen responded with a membrane hyperpolarization or an outward current. The hyperpolarization was dose-dependent, and the GABA(B) receptor antagonist CGP 35,348 produced a rightward shift in the agonist dose-response curve. Agonist potency was lower, and the efficacy greater, in GAD-positive neurons. The use of this novel technique for identifying GABAergic neurons thus reveals differences in the pharmacodynamics of GABA(B) receptor activation GABAergic and non- GABAergic ARC neurons.

Original languageEnglish (US)
Pages (from-to)4171-4177
Number of pages7
JournalNeuroReport
Volume9
Issue number18
StatePublished - Dec 21 1998

Fingerprint

GABA-B Receptors
GABAergic Neurons
Arcuate Nucleus of Hypothalamus
Neurons
GABA-B Receptor Antagonists
GABA-B Receptor Agonists
Baclofen
In Situ Hybridization
Guinea Pigs
Membranes

Keywords

  • Arcuate nucleus
  • Electrophysiology
  • GABA
  • Glutamatic acid decarboxylase
  • In situ hybridization
  • Potassium conductance

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Wagner, E. J., Bosch, M. A., Kelly, M., & Rønnekleiv, K. (1998). A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in the arcuate nucleus. NeuroReport, 9(18), 4171-4177.

A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in the arcuate nucleus. / Wagner, Edward J.; Bosch, Martha A.; Kelly, Martin; Rønnekleiv, K.

In: NeuroReport, Vol. 9, No. 18, 21.12.1998, p. 4171-4177.

Research output: Contribution to journalArticle

Wagner, EJ, Bosch, MA, Kelly, M & Rønnekleiv, K 1998, 'A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in the arcuate nucleus', NeuroReport, vol. 9, no. 18, pp. 4171-4177.
Wagner, Edward J. ; Bosch, Martha A. ; Kelly, Martin ; Rønnekleiv, K. / A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in the arcuate nucleus. In: NeuroReport. 1998 ; Vol. 9, No. 18. pp. 4171-4177.
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