A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in arcuate nucleus

Edward J. Wagner; Martha A. Bosch; Martin J. Kelly; Oline K. Rønnekleiv

doi:10.1097/00001756-199908200-00045

A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in arcuate nucleus

Edward J. Wagner, Martha A. Bosch, Martin J. Kelly, Oline K. Rønnekleiv

Physiology & Pharmacology

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We combined histofluorescence with in situ hybridization to identify GABAergic neurons in the arcuate nucleus (ARC) following electrophysiological recording, using GAD₆₅ as a marker. Intracellular recordings 91 were made in hypothalamic slices prepared from ovariectomized guinea pigs. Over 90% of ARC neurons tested with the GABA(B) receptor agonist baclofen responded with a membrane hyperpolarization or an outward current. The hyperpolarization was dose dependent, and the GABA(B) receptor antagonist CGP 35,348 produced a rightward shift in the agonist dose-response curve. Agonist potency was lower, and the efficacy greater, in GAD-positive neurons. The use of this novel technique for identifying GABAergic neurons thus reveals differences in the pharmacodynamics of GABA(B) receptor activation between GABAergic and non-GABAergic ARC neurons.

Original language	English (US)
Pages (from-to)	2681-2687
Number of pages	7
Journal	NeuroReport
Volume	10
Issue number	12
DOIs	https://doi.org/10.1097/00001756-199908200-00045
State	Published - Aug 20 1999

Keywords

Arcuate nucleus
Electrophysiology
Glutamic acid decarboxylase
In situ hybridization
Potassium conductance
γ-Aminobutyric acid

ASJC Scopus subject areas

Neuroscience(all)

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10.1097/00001756-199908200-00045

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abstract = "We combined histofluorescence with in situ hybridization to identify GABAergic neurons in the arcuate nucleus (ARC) following electrophysiological recording, using GAD65 as a marker. Intracellular recordings 91 were made in hypothalamic slices prepared from ovariectomized guinea pigs. Over 90% of ARC neurons tested with the GABA(B) receptor agonist baclofen responded with a membrane hyperpolarization or an outward current. The hyperpolarization was dose dependent, and the GABA(B) receptor antagonist CGP 35,348 produced a rightward shift in the agonist dose-response curve. Agonist potency was lower, and the efficacy greater, in GAD-positive neurons. The use of this novel technique for identifying GABAergic neurons thus reveals differences in the pharmacodynamics of GABA(B) receptor activation between GABAergic and non-GABAergic ARC neurons.",

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KW - Electrophysiology

KW - Glutamic acid decarboxylase

KW - In situ hybridization

KW - Potassium conductance

KW - γ-Aminobutyric acid

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A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in arcuate nucleus

Abstract

Keywords

ASJC Scopus subject areas

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