A position effect on the time of replication origin activation in yeast

Betsy M. Ferguson; Walton L. Fangman

doi:10.1016/0092-8674(92)90474-Q

A position effect on the time of replication origin activation in yeast

Betsy M. Ferguson, Walton L. Fangman

Research output: Contribution to journal › Article › peer-review

222 Scopus citations

Abstract

The chromosomes of eukaryotes are characterized by the mosaic nature of their replication - large regions of DNA that replicate early in S phase are interspersed with regions that replicate late. This pattern of early and late synthesis appears to be the consequence of a temporal program that activates replication origins at different times. The basis of this temporal regulation in the yeast S. cerevisiae has been investigated by changing the chromosomal locations of two origins, one activated early in the S phase (ARS1) and one activated late (ARS501). We show that the cis-acting information controlling time of activation can be separated from the element that determines origin function. For the ARS501 origin, late activation appears to be a consequence of its proximity to the telomere.

Original language	English (US)
Pages (from-to)	333-339
Number of pages	7
Journal	Cell
Volume	68
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(92)90474-Q
State	Published - Jan 24 1992
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(92)90474-Q

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title = "A position effect on the time of replication origin activation in yeast",

abstract = "The chromosomes of eukaryotes are characterized by the mosaic nature of their replication - large regions of DNA that replicate early in S phase are interspersed with regions that replicate late. This pattern of early and late synthesis appears to be the consequence of a temporal program that activates replication origins at different times. The basis of this temporal regulation in the yeast S. cerevisiae has been investigated by changing the chromosomal locations of two origins, one activated early in the S phase (ARS1) and one activated late (ARS501). We show that the cis-acting information controlling time of activation can be separated from the element that determines origin function. For the ARS501 origin, late activation appears to be a consequence of its proximity to the telomere.",

author = "Ferguson, {Betsy M.} and Fangman, {Walton L.}",

note = "Funding Information: We thank Bonita Brewer, Daniel Lockshon, and M. K. Raghuraman for their helpful suggestions on the manuscript. We thank Ann Reynolds for many helpful discussions during the course of this work and for providing materials for the experiment in Figure 4A. This work was supported by Public Health Service grant GM18926. B. M. F. was supported during part of the work by a PHS Training Grant.",

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doi = "10.1016/0092-8674(92)90474-Q",

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T1 - A position effect on the time of replication origin activation in yeast

AU - Ferguson, Betsy M.

AU - Fangman, Walton L.

N1 - Funding Information: We thank Bonita Brewer, Daniel Lockshon, and M. K. Raghuraman for their helpful suggestions on the manuscript. We thank Ann Reynolds for many helpful discussions during the course of this work and for providing materials for the experiment in Figure 4A. This work was supported by Public Health Service grant GM18926. B. M. F. was supported during part of the work by a PHS Training Grant.

PY - 1992/1/24

Y1 - 1992/1/24

N2 - The chromosomes of eukaryotes are characterized by the mosaic nature of their replication - large regions of DNA that replicate early in S phase are interspersed with regions that replicate late. This pattern of early and late synthesis appears to be the consequence of a temporal program that activates replication origins at different times. The basis of this temporal regulation in the yeast S. cerevisiae has been investigated by changing the chromosomal locations of two origins, one activated early in the S phase (ARS1) and one activated late (ARS501). We show that the cis-acting information controlling time of activation can be separated from the element that determines origin function. For the ARS501 origin, late activation appears to be a consequence of its proximity to the telomere.

AB - The chromosomes of eukaryotes are characterized by the mosaic nature of their replication - large regions of DNA that replicate early in S phase are interspersed with regions that replicate late. This pattern of early and late synthesis appears to be the consequence of a temporal program that activates replication origins at different times. The basis of this temporal regulation in the yeast S. cerevisiae has been investigated by changing the chromosomal locations of two origins, one activated early in the S phase (ARS1) and one activated late (ARS501). We show that the cis-acting information controlling time of activation can be separated from the element that determines origin function. For the ARS501 origin, late activation appears to be a consequence of its proximity to the telomere.

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A position effect on the time of replication origin activation in yeast

Abstract

ASJC Scopus subject areas

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