A Podospora anserina longevity mutant with a temperature-sensitive phenotype for senescence.

M. S. Turker, J. G. Nelson, D. J. Cummings

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Abstract

A Podospora anserina longevity mutant was identified with a temperature-sensitive phenotype for senescence. This mutant, termed TS1, grew for over 3 m at 27 degrees C, but when shifted to 34 degrees C, it underwent senescence between 10 and 18 cm. A previously described senescence-associated plasmid, alpha senDNA, derived from the mitochondrial genome, was not detected in TS1 at 27 degrees C but was present in senescent cultures at 34 degrees C. A similar result was observed in progeny strains obtained by crossing the TS1 mutant with a wild-type strain. Other mitochondrial excision-amplification DNAs in addition to alpha senDNA were also observed in the senescent cultures. Most were derived from a specific region of the mitochondrial genome. These results provide evidence that alpha senDNA is involved in TS1 senescence and suggest that this plasmid may play a role in the formation of other mitochondrial excision-amplification plasmids.

Original languageEnglish (US)
Pages (from-to)3199-3204
Number of pages6
JournalMolecular and cellular biology
Volume7
Issue number9
DOIs
StatePublished - Sep 1987

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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