A physical map of belomycin-specific fragmentation sites on PM2 bacteriophage DNA

R. Stephen Lloyd; Charles W. Haidle; Donald L. Robberson; Marion L. Dodson

doi:10.1007/BF02601707

A physical map of belomycin-specific fragmentation sites on PM2 bacteriophage DNA

R. Stephen Lloyd, Charles W. Haidle, Donald L. Robberson, Marion L. Dodson

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Bleomycin treatment of PM2 DNA results in fragmentation of the genome at several specific sites. Application of restriction endonuclease digestion followed by bleomycin treatment has provided the basis for constructing a physical map of bleomycin fragmentation sites. Eleven sites have been located on the physical map relative to Hpa II, Pst I, and HindIII cleavage sites. The fragmentation sites are not clustered in a particular region of the PM2 genome but 3 of the 11 sites do occur between the Hpa II and Pst I cleavage sites, a segment of DNA which comprises 14% of the PM2 DNA length.

Original language	English (US)
Pages (from-to)	45-50
Number of pages	6
Journal	Current Microbiology: An International Journal
Volume	1
Issue number	1
DOIs	https://doi.org/10.1007/BF02601707
State	Published - Jan 1978
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

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10.1007/BF02601707

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title = "A physical map of belomycin-specific fragmentation sites on PM2 bacteriophage DNA",

abstract = "Bleomycin treatment of PM2 DNA results in fragmentation of the genome at several specific sites. Application of restriction endonuclease digestion followed by bleomycin treatment has provided the basis for constructing a physical map of bleomycin fragmentation sites. Eleven sites have been located on the physical map relative to Hpa II, Pst I, and HindIII cleavage sites. The fragmentation sites are not clustered in a particular region of the PM2 genome but 3 of the 11 sites do occur between the Hpa II and Pst I cleavage sites, a segment of DNA which comprises 14% of the PM2 DNA length.",

author = "Lloyd, {R. Stephen} and Haidle, {Charles W.} and Robberson, {Donald L.} and Dodson, {Marion L.}",

year = "1978",

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AU - Lloyd, R. Stephen

AU - Haidle, Charles W.

AU - Robberson, Donald L.

AU - Dodson, Marion L.

PY - 1978/1

Y1 - 1978/1

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AB - Bleomycin treatment of PM2 DNA results in fragmentation of the genome at several specific sites. Application of restriction endonuclease digestion followed by bleomycin treatment has provided the basis for constructing a physical map of bleomycin fragmentation sites. Eleven sites have been located on the physical map relative to Hpa II, Pst I, and HindIII cleavage sites. The fragmentation sites are not clustered in a particular region of the PM2 genome but 3 of the 11 sites do occur between the Hpa II and Pst I cleavage sites, a segment of DNA which comprises 14% of the PM2 DNA length.

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A physical map of belomycin-specific fragmentation sites on PM2 bacteriophage DNA

Abstract

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