A phorbol ester-binding protein is required downstream of Rab5 in endosome fusion

Alejandro Aballay; M. Alejandro Barbieri; María I. Colombo; Graciela N. Arenas; Philip D. Stahl; Luis S. Mayorga

doi:10.1016/S0014-5793(98)01579-8

A phorbol ester-binding protein is required downstream of Rab5 in endosome fusion

Alejandro Aballay, M. Alejandro Barbieri, María I. Colombo, Graciela N. Arenas, Philip D. Stahl, Luis S. Mayorga

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Previous observations indicate that a zinc and phorbol ester binding factor is necessary for endosome fusion. To further characterize the role of this factor in the process, we used an in vitro endosome fusion assay supplemented with recombinant Rab5 proteins. Both zinc depletion and addition of calphostin C, an inhibitor of protein kinase C, inhibited endosome fusion in the presence of active Rab5. Addition of the phorbol ester PMA (phorbol 12-myristate 13-acetate) reversed the inhibition of endosome fusion caused by a Rab5 negative mutant. Moreover, PMA stimulated fusion in the presence of Rab5 immunodepleted cytosol. These results suggest that the phorbol ester binding protein is acting downstream of Rab5 in endosome fusion. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	373-378
Number of pages	6
Journal	FEBS Letters
Volume	441
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)01579-8
State	Published - Dec 28 1998
Externally published	Yes

Keywords

Endocytosis
Endosome fusion
Phorbol ester
Protein kinase C
Rab5

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(98)01579-8

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title = "A phorbol ester-binding protein is required downstream of Rab5 in endosome fusion",

abstract = "Previous observations indicate that a zinc and phorbol ester binding factor is necessary for endosome fusion. To further characterize the role of this factor in the process, we used an in vitro endosome fusion assay supplemented with recombinant Rab5 proteins. Both zinc depletion and addition of calphostin C, an inhibitor of protein kinase C, inhibited endosome fusion in the presence of active Rab5. Addition of the phorbol ester PMA (phorbol 12-myristate 13-acetate) reversed the inhibition of endosome fusion caused by a Rab5 negative mutant. Moreover, PMA stimulated fusion in the presence of Rab5 immunodepleted cytosol. These results suggest that the phorbol ester binding protein is acting downstream of Rab5 in endosome fusion. Copyright (C) 1998 Federation of European Biochemical Societies.",

keywords = "Endocytosis, Endosome fusion, Phorbol ester, Protein kinase C, Rab5",

author = "Alejandro Aballay and Barbieri, {M. Alejandro} and Colombo, {Mar{\'i}a I.} and Arenas, {Graciela N.} and Stahl, {Philip D.} and Mayorga, {Luis S.}",

note = "Funding Information: This work was partly supported by an International Research Scholar Award from the Howard Hughes Medical Institute, by grants from the National Institutes of Health, the National Science Foundation-CONICET International Program, CONICET, CIUNC, and Fundaci{\'o}n Antorchas. A.A. is supported by Pew and CONICET fellowships.",

year = "1998",

month = dec,

day = "28",

doi = "10.1016/S0014-5793(98)01579-8",

language = "English (US)",

volume = "441",

pages = "373--378",

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number = "3",

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TY - JOUR

T1 - A phorbol ester-binding protein is required downstream of Rab5 in endosome fusion

AU - Aballay, Alejandro

AU - Barbieri, M. Alejandro

AU - Colombo, María I.

AU - Arenas, Graciela N.

AU - Stahl, Philip D.

AU - Mayorga, Luis S.

N1 - Funding Information: This work was partly supported by an International Research Scholar Award from the Howard Hughes Medical Institute, by grants from the National Institutes of Health, the National Science Foundation-CONICET International Program, CONICET, CIUNC, and Fundación Antorchas. A.A. is supported by Pew and CONICET fellowships.

PY - 1998/12/28

Y1 - 1998/12/28

N2 - Previous observations indicate that a zinc and phorbol ester binding factor is necessary for endosome fusion. To further characterize the role of this factor in the process, we used an in vitro endosome fusion assay supplemented with recombinant Rab5 proteins. Both zinc depletion and addition of calphostin C, an inhibitor of protein kinase C, inhibited endosome fusion in the presence of active Rab5. Addition of the phorbol ester PMA (phorbol 12-myristate 13-acetate) reversed the inhibition of endosome fusion caused by a Rab5 negative mutant. Moreover, PMA stimulated fusion in the presence of Rab5 immunodepleted cytosol. These results suggest that the phorbol ester binding protein is acting downstream of Rab5 in endosome fusion. Copyright (C) 1998 Federation of European Biochemical Societies.

AB - Previous observations indicate that a zinc and phorbol ester binding factor is necessary for endosome fusion. To further characterize the role of this factor in the process, we used an in vitro endosome fusion assay supplemented with recombinant Rab5 proteins. Both zinc depletion and addition of calphostin C, an inhibitor of protein kinase C, inhibited endosome fusion in the presence of active Rab5. Addition of the phorbol ester PMA (phorbol 12-myristate 13-acetate) reversed the inhibition of endosome fusion caused by a Rab5 negative mutant. Moreover, PMA stimulated fusion in the presence of Rab5 immunodepleted cytosol. These results suggest that the phorbol ester binding protein is acting downstream of Rab5 in endosome fusion. Copyright (C) 1998 Federation of European Biochemical Societies.

KW - Endocytosis

KW - Endosome fusion

KW - Phorbol ester

KW - Protein kinase C

KW - Rab5

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AN - SCOPUS:0032443397

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JO - FEBS Letters

JF - FEBS Letters

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ER -

A phorbol ester-binding protein is required downstream of Rab5 in endosome fusion

Abstract

Keywords

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