TY - JOUR
T1 - A phase II randomized placebo-controlled trial of oral n-acetylcysteine for protection of melanocytic nevi against uv-induced oxidative stress in vivo
AU - Cassidy, Pamela B.
AU - Liu, Tong
AU - Florell, Scott R.
AU - Honeggar, Matthew
AU - Leachman, Sancy A.
AU - Boucher, Kenneth M.
AU - Grossman, Douglas
N1 - Publisher Copyright:
© 2017 American Association for Cancer Research.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Oxidative stress plays a role in UV-induced melanoma, which may arise from melanocytic nevi. We investigated whether oral administration of the antioxidant N-acetylcysteine (NAC) could protect nevi from oxidative stress in vivo in the setting of acute UV exposure. The minimal erythemal dose (MED) was determined for 100 patients at increased risk for melanoma. Patients were randomized to receive a single dose (1,200 mg) of NAC or placebo, in double-blind fashion, and then one nevus was irradiated (1-2 MED) using a solar simulator. One day later, theMED was redetermined and the irradiated nevus and a control unirradiated nevus were removed for histologic analysis and examination of biomarkers ofNACmetabolism and UV-induced oxidative stress. Increased expression of 8-oxoguanine, thioredoxin reductase- 1, and g-glutamylcysteine synthase modifier subunit were consistently seen in UV-treated compared with unirradiated nevi. However, no significant differences were observed in these UVinduced changes or in the pre- and postintervention MED between those patients receiving NAC versus placebo. Similarly, no significant differences were observed in UV-induced changes between subjects with germline wild-type versus loss-of-function mutations in the melanocortin-1 receptor. Nevi showed similar changes of UV-induced oxidative stress in an open-label post-trial study in 10 patients who received NAC 3 hours before nevus irradiation. Thus, a single oral dose of NAC did not effectively protect nevi from UV-induced oxidative stress under the conditions examined.
AB - Oxidative stress plays a role in UV-induced melanoma, which may arise from melanocytic nevi. We investigated whether oral administration of the antioxidant N-acetylcysteine (NAC) could protect nevi from oxidative stress in vivo in the setting of acute UV exposure. The minimal erythemal dose (MED) was determined for 100 patients at increased risk for melanoma. Patients were randomized to receive a single dose (1,200 mg) of NAC or placebo, in double-blind fashion, and then one nevus was irradiated (1-2 MED) using a solar simulator. One day later, theMED was redetermined and the irradiated nevus and a control unirradiated nevus were removed for histologic analysis and examination of biomarkers ofNACmetabolism and UV-induced oxidative stress. Increased expression of 8-oxoguanine, thioredoxin reductase- 1, and g-glutamylcysteine synthase modifier subunit were consistently seen in UV-treated compared with unirradiated nevi. However, no significant differences were observed in these UVinduced changes or in the pre- and postintervention MED between those patients receiving NAC versus placebo. Similarly, no significant differences were observed in UV-induced changes between subjects with germline wild-type versus loss-of-function mutations in the melanocortin-1 receptor. Nevi showed similar changes of UV-induced oxidative stress in an open-label post-trial study in 10 patients who received NAC 3 hours before nevus irradiation. Thus, a single oral dose of NAC did not effectively protect nevi from UV-induced oxidative stress under the conditions examined.
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U2 - 10.1158/1940-6207.CAPR-16-0162
DO - 10.1158/1940-6207.CAPR-16-0162
M3 - Article
C2 - 27920018
AN - SCOPUS:85009212390
SN - 1940-6207
VL - 10
SP - 36
EP - 44
JO - Cancer Prevention Research
JF - Cancer Prevention Research
IS - 1
ER -