A perfusion system developed for 31p NMR study of melanoma cells at tissue‐like density

Margaret M. Minichiello, Daniel M. Albert, Nancy H. Kolodny, Moon‐Soo ‐S Lee, Joseph L. Craft

Research output: Contribution to journalComment/debate

10 Scopus citations

Abstract

A perfusion culture system has been developed for 31P NMR study of human uveal melanoma metabolism by adapting the Vitafiber I cartridge system (Amicon). 31P NMR spectra collected weekly during periods of up to 10 weeks demonstrated increasing levels of phosphorus metabolites as the anchorage‐dependent cells grew to tissue‐like density. © 1989 Academic Press, Inc.

Original languageEnglish (US)
Pages (from-to)96-107
Number of pages12
JournalMagnetic Resonance in Medicine
Volume10
Issue number1
DOIs
StatePublished - Apr 1989

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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