A perfusion culture system has been developed for 31P NMR study of human uveal melanoma metabolism by adapting the Vitafiber I cartridge system (Amicon). 31P NMR spectra collected weekly during periods of up to 10 weeks demonstrated increasing levels of phosphorus metabolites as the anchorage‐dependent cells grew to tissue‐like density. © 1989 Academic Press, Inc.
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging