A paravascular pathway facilitates CSF flow through the brain parenchyma and the clearance of interstitial solutes, including amyloid β

Jeffrey Iliff, Minghuan Wang, Yonghong Liao, Benjamin A. Plogg, Weiguo Peng, Georg A. Gundersen, Helene Benveniste, G. Edward Vates, Rashid Deane, Steven A. Goldman, Erlend A. Nagelhus, Maiken Nedergaard

Research output: Contribution to journalArticle

1294 Citations (Scopus)

Abstract

Because it lacks a lymphatic circulation, the brain must clear extracellular proteins by an alternative mechanism. The cerebrospinal fluid (CSF) functions as a sink for brain extracellular solutes, but it is not clear how solutes from the brain interstitium move from the parenchyma to the CSF. We demonstrate that a substantial portion of subarachnoid CSF cycles through the brain interstitial space. On the basis of in vivo two-photon imaging of small fluorescent tracers, we showed that CSF enters the parenchyma along paravascular spaces that surround penetrating arteries and that brain interstitial fluid is cleared along paravenous drainage pathways. Animals lacking the water channel aquaporin-4 (AQP4) in astrocytes exhibit slowed CSF influx through this system and a ∼70% reduction in interstitial solute clearance, suggesting that the bulk fluid flow between these anatomical influx and efflux routes is supported by astrocytic water transport. Fluorescent-tagged amyloid β, a peptide thought to be pathogenic in Alzheimer's disease, was transported along this route, and deletion of the Aqp4 gene suppressed the clearance of soluble amyloid β, suggesting that this pathway may remove amyloid βfrom the central nervous system. Clearance through paravenous flow may also regulate extracellular levels of proteins involved with neurodegenerative conditions, its impairment perhaps contributing to the mis-accumulation of soluble proteins.

Original languageEnglish (US)
Article number147ra111
JournalScience Translational Medicine
Volume4
Issue number147
DOIs
StatePublished - Aug 15 2012
Externally publishedYes

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Amyloid
Cerebrospinal Fluid
Brain
Aquaporin 4
Aquaporins
Proteins
Extracellular Fluid
Gene Deletion
Photons
Astrocytes
Drainage
Alzheimer Disease
Central Nervous System
Arteries
Peptides
Water

ASJC Scopus subject areas

  • Medicine(all)

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A paravascular pathway facilitates CSF flow through the brain parenchyma and the clearance of interstitial solutes, including amyloid β. / Iliff, Jeffrey; Wang, Minghuan; Liao, Yonghong; Plogg, Benjamin A.; Peng, Weiguo; Gundersen, Georg A.; Benveniste, Helene; Vates, G. Edward; Deane, Rashid; Goldman, Steven A.; Nagelhus, Erlend A.; Nedergaard, Maiken.

In: Science Translational Medicine, Vol. 4, No. 147, 147ra111, 15.08.2012.

Research output: Contribution to journalArticle

Iliff, J, Wang, M, Liao, Y, Plogg, BA, Peng, W, Gundersen, GA, Benveniste, H, Vates, GE, Deane, R, Goldman, SA, Nagelhus, EA & Nedergaard, M 2012, 'A paravascular pathway facilitates CSF flow through the brain parenchyma and the clearance of interstitial solutes, including amyloid β', Science Translational Medicine, vol. 4, no. 147, 147ra111. https://doi.org/10.1126/scitranslmed.3003748
Iliff, Jeffrey ; Wang, Minghuan ; Liao, Yonghong ; Plogg, Benjamin A. ; Peng, Weiguo ; Gundersen, Georg A. ; Benveniste, Helene ; Vates, G. Edward ; Deane, Rashid ; Goldman, Steven A. ; Nagelhus, Erlend A. ; Nedergaard, Maiken. / A paravascular pathway facilitates CSF flow through the brain parenchyma and the clearance of interstitial solutes, including amyloid β. In: Science Translational Medicine. 2012 ; Vol. 4, No. 147.
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