A panel of 20 highly variable microsatellite polymorphisms in rhesus macaques (Macaca mulatta) selected for pedigree or population genetic analysis

Jeffrey Rogers, Mackenzie Bergstrom, Roy Garcia IV, Jordan Kaplan, Ann Arya, Leilah Novakowski, Zach Johnson, Amanda Vinson, Wendy Shelledy

    Research output: Contribution to journalArticlepeer-review

    15 Scopus citations

    Abstract

    This paper reports 20 new microsatellite loci that are highly polymorphic in rhesus macaques (Macaca mulatta). We screened known human microsatellite loci to identify markers that are polymorphic in rhesus macaques, and then selected specific loci that show substantial levels of heterozygosity and robust, reliable amplification. The 20 loci reported here were chosen to include one highly informative microsatellite from each rhesus monkey autosomal chromosome. Fourteen of the 20 polymorphisms are tetranucleotide repeats, and all can be analyzed using standard PCR and electrophoresis procedures. These new rhesus markers have an average of 15.5 alleles per locus and average heterozygosity of 0.83. This panel of DNA polymorphisms will be useful for a variety of different genetic analyses, including pedigree testing, paternity analysis, and population genetic studies. Many of these loci are also likely to be informative in other closely related Old World monkey species.

    Original languageEnglish (US)
    Pages (from-to)377-383
    Number of pages7
    JournalAmerican Journal of Primatology
    Volume67
    Issue number3
    DOIs
    StatePublished - Nov 2005

    Keywords

    • Microsatellite
    • Polymorphism
    • Rhesus macaque
    • STR
    • Tetranucleotide

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Animal Science and Zoology

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