A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs

Avathamsa Athirasala, Fernanda Lins, Anthony Tahayeri, Monica Hinds, Anthony J. Smith, Christine Sedgley, Jack Ferracane, Luiz E. Bertassoni

Research output: Research - peer-reviewArticle

Abstract

The requirement for immediate vascularization of engineered dental pulp poses a major hurdle towards successful implementation of pulp regeneration as an effective therapeutic strategy for root canal therapy, especially in adult teeth. Here, we demonstrate a novel strategy to engineer pre-vascularized, cell-laden hydrogel pulp-like tissue constructs in full-length root canals for dental pulp regeneration. We utilized gelatin methacryloyl (GelMA) hydrogels with tunable physical and mechanical properties to determine the microenvironmental conditions (microstructure, degradation, swelling and elastic modulus) that enhanced viability, spreading and proliferation of encapsulated odontoblast-like cells (OD21), and the formation of endothelial monolayers by endothelial colony forming cells (ECFCs). GelMA hydrogels with higher polymer concentration (15% w/v) and stiffness enhanced OD21 cell viability, spreading and proliferation, as well as endothelial cell spreading and monolayer formation. We then fabricated pre-vascularized, full-length, dental pulp-like tissue constructs by dispensing OD21 cell-laden GelMA hydrogel prepolymer in root canals of extracted teeth and fabricating 500 μm channels throughout the root canals. ECFCs seeded into the microchannels successfully formed monolayers and underwent angiogenic sprouting within 7 days in culture. In summary, the proposed approach is a simple and effective strategy for engineering of pre-vascularized dental pulp constructs offering potentially beneficial translational outcomes.

LanguageEnglish (US)
Article number3323
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Dental Pulp
Dental Pulp Cavity
Gelatin
Hydrogels
Hydrogel
Regeneration
Tooth
Root Canal Therapy
Odontoblasts
Elastic Modulus
Cell Survival
Polymers
Endothelial Cells
Cell Proliferation
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs. / Athirasala, Avathamsa; Lins, Fernanda; Tahayeri, Anthony; Hinds, Monica; Smith, Anthony J.; Sedgley, Christine; Ferracane, Jack; Bertassoni, Luiz E.

In: Scientific Reports, Vol. 7, No. 1, 3323, 01.12.2017.

Research output: Research - peer-reviewArticle

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