A novel signature mutation for oxidative damage resembles a mutational pattern found commonly in human cancers

Mitchell Turker, Blythe M. Gage, Jennifer A. Rose, Daniel Elroy, Olga N. Ponomareva, Peter J. Stambrook, Jay A. Tischfield

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

To determine the types of mutations induced by oxidative damage, a kidney cell line with a heterozygous deficiency for the autosomal Aprt (adenine phosphoribosyltransferase) gene was tested for its mutagenic response to hydrogen peroxide. Aprt-deficient cells were selected and scored for loss of heterozygosity (LOH) for 11 microsatellite loci on mouse chromosome 8. On the basis of the LOH analysis, spontaneous mutants (n = 38) were distributed into four classes: apparent point mutation, mitotic recombination, chromosome loss, and large interstitial deletion. However, 9 of 20 (45%) hydrogen peroxide-induced mutants exhibited a novel class of mutations characterized by 'discontinuous LOH' for one or more of the microsatellite loci. Interestingly, mutations resembling discontinuous LOH are commonly observed in a wide variety of human cancers. Our data suggest that discontinuous LOH is a signature mutational pattern for oxidative damage and further suggest that such genetic damage is widespread in cancer.

Original languageEnglish (US)
Pages (from-to)1837-1839
Number of pages3
JournalCancer Research
Volume59
Issue number8
StatePublished - Apr 15 1999

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Loss of Heterozygosity
Mutation
Neoplasms
Microsatellite Repeats
Hydrogen Peroxide
Adenine Phosphoribosyltransferase
Chromosomes, Human, Pair 8
Point Mutation
Genetic Recombination
Chromosomes
Kidney
Cell Line
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Turker, M., Gage, B. M., Rose, J. A., Elroy, D., Ponomareva, O. N., Stambrook, P. J., & Tischfield, J. A. (1999). A novel signature mutation for oxidative damage resembles a mutational pattern found commonly in human cancers. Cancer Research, 59(8), 1837-1839.

A novel signature mutation for oxidative damage resembles a mutational pattern found commonly in human cancers. / Turker, Mitchell; Gage, Blythe M.; Rose, Jennifer A.; Elroy, Daniel; Ponomareva, Olga N.; Stambrook, Peter J.; Tischfield, Jay A.

In: Cancer Research, Vol. 59, No. 8, 15.04.1999, p. 1837-1839.

Research output: Contribution to journalArticle

Turker, M, Gage, BM, Rose, JA, Elroy, D, Ponomareva, ON, Stambrook, PJ & Tischfield, JA 1999, 'A novel signature mutation for oxidative damage resembles a mutational pattern found commonly in human cancers', Cancer Research, vol. 59, no. 8, pp. 1837-1839.
Turker M, Gage BM, Rose JA, Elroy D, Ponomareva ON, Stambrook PJ et al. A novel signature mutation for oxidative damage resembles a mutational pattern found commonly in human cancers. Cancer Research. 1999 Apr 15;59(8):1837-1839.
Turker, Mitchell ; Gage, Blythe M. ; Rose, Jennifer A. ; Elroy, Daniel ; Ponomareva, Olga N. ; Stambrook, Peter J. ; Tischfield, Jay A. / A novel signature mutation for oxidative damage resembles a mutational pattern found commonly in human cancers. In: Cancer Research. 1999 ; Vol. 59, No. 8. pp. 1837-1839.
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