A novel role of the interferon-inducible protein IFI16 as inducer of proinflammatory molecules in endothelial cells

Patrizia Caposio, Francesca Gugliesi, Claudia Zannetti, Simone Sponza, Michele Mondini, Enzo Medico, John Hiscott, Howard A. Young, Giorgio Gribaudo, Marisa Gariglio, Santo Landolfo

Research output: Contribution to journalArticle

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Abstract

The human IFI16 gene is an interferon-inducible gene implicated in the regulation of endothelial cell proliferation and tube morphogenesis. Immunohistochemical analysis has demonstrated that this gene is highly expressed in endothelial cells in addition to hematopoietic tissues. In this study, gene array analysis of human umbilical vein endothelial cells overexpressing IFI16 revealed an increased expression of genes involved in immunomodulation, cell growth, and apoptosis. Consistent with these observations, IFI16 triggered expression of adhesion molecules such as ICAM-1 and E-selectin or chemokines such as interleukin-8 or MCP-1. Treatment of cells with short hairpin RNA targeting IFI16 significantly inhibited ICAM-1 induction by interferon (IFN)-γ demonstrating that IFI16 is required for proinflammatory gene stimulation. Moreover, functional analysis of the ICAM-1 promoter by deletion- or site-specific mutation demonstrated that NF-κB is the main mediator of IFI16-driven gene induction. NF-κB activation appears to be triggered by IFI16 through a novel mechanism involving suppression of IκBα mRNA and protein expression. Support for this finding comes from the observation that IFI16 targeting with specific short hairpin RNA down-regulates NF-κB binding activity to its cognate DNA and inhibits ICAM-1 expression induced by IFN-γ. Using transient transfection and luciferase assay, electrophoretic mobility shift assay, and chromatin immunoprecipitation, we demonstrate indeed that activation of the NF-κB response is mediated by IFI16-induced block of Sp1-like factor recruitment to the promoter of the IκBα gene, encoding the main NF-κB inhibitor. Activation of NF-κB accompanied by induction of proinflammatory molecules was also observed when IκBα expression was down-regulated by specific small interfering RNA, resulting in an outcome similar to that observed with IFI16 overexpression. Taken together, these data implicate IFI16 as a novel regulator of endothelial proinflammatory activity and provide new insights into the physiological functions of the IFN-inducible gene IFI16.

Original languageEnglish (US)
Pages (from-to)33515-33529
Number of pages15
JournalJournal of Biological Chemistry
Volume282
Issue number46
DOIs
StatePublished - Nov 16 2007
Externally publishedYes

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Endothelial cells
Interferons
Endothelial Cells
Genes
Molecules
Intercellular Adhesion Molecule-1
Proteins
Small Interfering RNA
Chemical activation
Assays
Electrophoretic mobility
Functional analysis
Gene encoding
E-Selectin
Immunomodulation
Chromatin Immunoprecipitation
Human Umbilical Vein Endothelial Cells
Cell proliferation
Cell growth
Electrophoretic Mobility Shift Assay

ASJC Scopus subject areas

  • Biochemistry

Cite this

A novel role of the interferon-inducible protein IFI16 as inducer of proinflammatory molecules in endothelial cells. / Caposio, Patrizia; Gugliesi, Francesca; Zannetti, Claudia; Sponza, Simone; Mondini, Michele; Medico, Enzo; Hiscott, John; Young, Howard A.; Gribaudo, Giorgio; Gariglio, Marisa; Landolfo, Santo.

In: Journal of Biological Chemistry, Vol. 282, No. 46, 16.11.2007, p. 33515-33529.

Research output: Contribution to journalArticle

Caposio, P, Gugliesi, F, Zannetti, C, Sponza, S, Mondini, M, Medico, E, Hiscott, J, Young, HA, Gribaudo, G, Gariglio, M & Landolfo, S 2007, 'A novel role of the interferon-inducible protein IFI16 as inducer of proinflammatory molecules in endothelial cells', Journal of Biological Chemistry, vol. 282, no. 46, pp. 33515-33529. https://doi.org/10.1074/jbc.M701846200
Caposio, Patrizia ; Gugliesi, Francesca ; Zannetti, Claudia ; Sponza, Simone ; Mondini, Michele ; Medico, Enzo ; Hiscott, John ; Young, Howard A. ; Gribaudo, Giorgio ; Gariglio, Marisa ; Landolfo, Santo. / A novel role of the interferon-inducible protein IFI16 as inducer of proinflammatory molecules in endothelial cells. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 46. pp. 33515-33529.
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abstract = "The human IFI16 gene is an interferon-inducible gene implicated in the regulation of endothelial cell proliferation and tube morphogenesis. Immunohistochemical analysis has demonstrated that this gene is highly expressed in endothelial cells in addition to hematopoietic tissues. In this study, gene array analysis of human umbilical vein endothelial cells overexpressing IFI16 revealed an increased expression of genes involved in immunomodulation, cell growth, and apoptosis. Consistent with these observations, IFI16 triggered expression of adhesion molecules such as ICAM-1 and E-selectin or chemokines such as interleukin-8 or MCP-1. Treatment of cells with short hairpin RNA targeting IFI16 significantly inhibited ICAM-1 induction by interferon (IFN)-γ demonstrating that IFI16 is required for proinflammatory gene stimulation. Moreover, functional analysis of the ICAM-1 promoter by deletion- or site-specific mutation demonstrated that NF-κB is the main mediator of IFI16-driven gene induction. NF-κB activation appears to be triggered by IFI16 through a novel mechanism involving suppression of IκBα mRNA and protein expression. Support for this finding comes from the observation that IFI16 targeting with specific short hairpin RNA down-regulates NF-κB binding activity to its cognate DNA and inhibits ICAM-1 expression induced by IFN-γ. Using transient transfection and luciferase assay, electrophoretic mobility shift assay, and chromatin immunoprecipitation, we demonstrate indeed that activation of the NF-κB response is mediated by IFI16-induced block of Sp1-like factor recruitment to the promoter of the IκBα gene, encoding the main NF-κB inhibitor. Activation of NF-κB accompanied by induction of proinflammatory molecules was also observed when IκBα expression was down-regulated by specific small interfering RNA, resulting in an outcome similar to that observed with IFI16 overexpression. Taken together, these data implicate IFI16 as a novel regulator of endothelial proinflammatory activity and provide new insights into the physiological functions of the IFN-inducible gene IFI16.",
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AU - Sponza, Simone

AU - Mondini, Michele

AU - Medico, Enzo

AU - Hiscott, John

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AU - Landolfo, Santo

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