A novel noninvasive method for evaluating experimental lung metastasis in mice

Arnulfo Mendoza, Radhika Gharpure, John Dennis, Joshua D. Webster, Jeremy Smedley, Chand Khanna

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Metastasis remains the most significant problem in the field of cancer. The biologic complexity that characterizes metastasis requires relevant in vivo models. When using murine models for pulmonary metastasis, longitudinal studies are valuable for following the progression of metastatic burden. Currently, the progression of pulmonary metastatic burden in experimental mice over time is monitored through advanced imaging approaches or the clinical assessment of morbidity. Because clinical signs of morbidity are often vague and unpredictable, an inexpensive and reproducible method to detect advanced metastatic burden - before the development of mortality - is needed. We have developed a noninvasive technique for assessing pulmonary metastatic burden in laboratory mice. The pulmonary assessment of advanced metastasis (PAAM) test is performed by restraining an awake mouse and gently applying pressure with the index finger under the xiphoid process. This pressure reduces the diaphragmatic component to respiration. Mice with advanced lung metastases show transient signs of respiratory distress within 3 s of the application of this pressure. Using PAAM in 4 distinct models (including sarcoma and mammary carcinoma histologies) of experimental (tail vein) pulmonary metastasis (n = 114 mice), among 3 independent evaluators yielded 94% positive and negative predictive values, which were validated by histologic assessment of postmortem lung tissue. PAAM is a simple, reproducible, and efficient method to assist in the detection of advanced pulmonary metastasis in mice and contributes to their humane care during longitudinal studies.

Original languageEnglish (US)
Pages (from-to)584-589
Number of pages6
JournalJournal of the American Association for Laboratory Animal Science
Volume52
Issue number5
StatePublished - Sep 1 2013
Externally publishedYes

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metastasis
lungs
mice
methodology
longitudinal studies
morbidity
sarcoma
distress
breast neoplasms
histology
breathing
animal models
image analysis
neoplasms

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

A novel noninvasive method for evaluating experimental lung metastasis in mice. / Mendoza, Arnulfo; Gharpure, Radhika; Dennis, John; Webster, Joshua D.; Smedley, Jeremy; Khanna, Chand.

In: Journal of the American Association for Laboratory Animal Science, Vol. 52, No. 5, 01.09.2013, p. 584-589.

Research output: Contribution to journalArticle

Mendoza, Arnulfo ; Gharpure, Radhika ; Dennis, John ; Webster, Joshua D. ; Smedley, Jeremy ; Khanna, Chand. / A novel noninvasive method for evaluating experimental lung metastasis in mice. In: Journal of the American Association for Laboratory Animal Science. 2013 ; Vol. 52, No. 5. pp. 584-589.
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