A Novel Model of Bacterially-Induced Acute Anterior Uveitis in Rats and the Lack of Effect from HLA-B27 Expression

Safia Baggia, Jennifer L. Lyons, Ethan Angell, Andre Barkhuizen, Young Bock Han, Stephen Planck, Joel D. Taurog, James (Jim) Rosenbaum

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Humans with the major histocompatibility antigen B27 (HLA-B27) are especially at risk for developing rheumatic disorders such as ankylosing spondylitis and Reiter's syndrome. Acute anterior uveitis (AAU) often occurs in association with these diseases or in HLA B27 positive individuals without joint disease. Methods: We induced acute anterior uveitis in Lewis rats by a standard model, the intraperitoneal injection of 200 μg of Escherichia coli endotoxin. We also developed a novel model of uveitis secondary to gram-negative infection. Results: Transgenic rats that expressed a low copy number of the B27 gene did not differ statistically from litter mate controls in the intensity of anterior uveitis as judged by histology, enumeration of cells in aqueous humor, protein in aqueous humor, or slit lamp examination. The majority of rats exposed to live Salmonella enteritidis or Yersinia enterocolitica 0:3 using either an oral or intravenous route of infection developed anterior uveitis. In contrast to the disease induced by endotoxin that is most intense 24 hours after the endotoxin challenge, uveitis induced by live bacteria usually began 7 to 9 days after exposure to bacterial products, was more often unilateral, persisted for as long as 3 weeks, and was sometimes recurrent. The expression of HLA-B27 did not appear to influence the incidence or severity of uveitis in B27+ low copy heterozygous animals. Conclusion: This rat model of AAU should facilitate evaluation of bacterial antigenic component(s) involved in the pathogenesis of live gram-negative bacteria induced AAU.

Original languageEnglish (US)
Pages (from-to)295-301
Number of pages7
JournalJournal of Investigative Medicine
Volume45
Issue number5
StatePublished - Jun 1997

Fingerprint

Anterior Uveitis
Histocompatibility Antigens
Rats
Uveitis
Endotoxins
Bacteria
Aqueous Humor
HLA-B27 Antigen
Salmonella
Histology
Electric lamps
Transgenic Rats
Reactive Arthritis
Salmonella enteritidis
Yersinia enterocolitica
Animals
Gene Dosage
Joint Diseases
Genes
Ankylosing Spondylitis

Keywords

  • Anterior uveitis
  • Endotoxin
  • Gram-negative bacteria
  • HLA-B27
  • Transgenic

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A Novel Model of Bacterially-Induced Acute Anterior Uveitis in Rats and the Lack of Effect from HLA-B27 Expression. / Baggia, Safia; Lyons, Jennifer L.; Angell, Ethan; Barkhuizen, Andre; Han, Young Bock; Planck, Stephen; Taurog, Joel D.; Rosenbaum, James (Jim).

In: Journal of Investigative Medicine, Vol. 45, No. 5, 06.1997, p. 295-301.

Research output: Contribution to journalArticle

Baggia, Safia ; Lyons, Jennifer L. ; Angell, Ethan ; Barkhuizen, Andre ; Han, Young Bock ; Planck, Stephen ; Taurog, Joel D. ; Rosenbaum, James (Jim). / A Novel Model of Bacterially-Induced Acute Anterior Uveitis in Rats and the Lack of Effect from HLA-B27 Expression. In: Journal of Investigative Medicine. 1997 ; Vol. 45, No. 5. pp. 295-301.
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