A novel intermediate filament-associated protein, NAPA-73, that binds to different filament types at different stages of nervous system development

Gary Ciment, A. Ressler, P. C. Letourneau, J. A. Weston

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The antigen recognized by the E/C8-monoclonal antibody is expressed in various avian embryonic cell types known also to express neurofilament (NF) immunoreactivity. To determine whether the E/C8-antigen corresponds to any of the known NF components, we compared their subcellular locations, immunocross-reactivities, and electrophoretic behaviors. We found that the E/C8-antibody binds to NF bundles in electron microscope preparations of neurons, but does not correspond to any of the known NF proteins by immunological or electrophoretic criteria. Immunoadsorption with the monoclonal antibody resulted in co-purification of a 73,000-D protein with one of the known NF proteins in homogenates from 20-d embryonic chick brains, but with vimentin intermediate filament protein in similarly prepared homogenates from 4-d embryonic chicks. We suggest that the E/C8-antigen is an intermediate filament-associated protein that binds to different filament types at different stages of development. We have named it NAPA-73, an acronym for neurofilament-associated protein, avian-specific, 73,000 D, on the basis of its binding specificity in mature neurons.

Original languageEnglish (US)
Pages (from-to)246-251
Number of pages6
JournalJournal of Cell Biology
Volume102
Issue number1
StatePublished - 1986
Externally publishedYes

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Neurofilament Proteins
Intermediate Filament Proteins
Intermediate Filaments
Nervous System
Antigens
Monoclonal Antibodies
Neurons
Vimentin
Electrons
Antibodies
Brain
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

A novel intermediate filament-associated protein, NAPA-73, that binds to different filament types at different stages of nervous system development. / Ciment, Gary; Ressler, A.; Letourneau, P. C.; Weston, J. A.

In: Journal of Cell Biology, Vol. 102, No. 1, 1986, p. 246-251.

Research output: Contribution to journalArticle

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