A novel human insulin-like growth factor I messenger RNA is expressed in normal and tumor cells

Garry Tobin, Douglas Yee, Nils Brünner, Peter Rotwein

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

We have identified and characterized a novel human insulin-like growth factor I (IGF-I) precursor from the transplantable T61 human breast cancer xenograft and from normal liver. The mRNA encoding this precursor contains a 5′-untranslated region that is 83% identical to the corresponding region of a previously described variant rat IGF-I. The nucleotide sequence of the cloned cDNA predicts an IGF-IA protein precursor of 137 amino acids, including a 32 residue signal peptide, 70 amino acid IGF-I, and a 35 residue COOH-terminal extension or E peptide. The exon encoding this variant maps in the genome between IGF-I exons 1 and 2, in a similar location to the homologous rat exon 1a. The rat and human exons 1a are 59% identical over 1443 nucleotides, with DNA sequence conservation occurring in a mosaic pattern. Human IGF-I mRNAs encoding this novel exon are expressed in liver, T61 tumor cells, and in an ovarian carcinoma cell line, NIH OVCAR3. These studies demonstrate that as in the rat, the human IGF-I gene contains six exons that are variably processed into multiple IGF-I mRNAs. The mechanisms responsible for generating different IGF-I mRNAs thus appear to be conserved among mammalian species.

Original languageEnglish (US)
Pages (from-to)1914-1920
Number of pages7
JournalMolecular Endocrinology
Volume4
Issue number12
StatePublished - 1990
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor I
Exons
Messenger RNA
Neoplasms
Amino Acids
Protein Precursors
immune RNA
5' Untranslated Regions
Liver
RNA Precursors
Protein Sorting Signals
Heterografts
Nucleotides
Complementary DNA
Genome
Breast Neoplasms
Carcinoma
Cell Line
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

A novel human insulin-like growth factor I messenger RNA is expressed in normal and tumor cells. / Tobin, Garry; Yee, Douglas; Brünner, Nils; Rotwein, Peter.

In: Molecular Endocrinology, Vol. 4, No. 12, 1990, p. 1914-1920.

Research output: Contribution to journalArticle

Tobin, G, Yee, D, Brünner, N & Rotwein, P 1990, 'A novel human insulin-like growth factor I messenger RNA is expressed in normal and tumor cells', Molecular Endocrinology, vol. 4, no. 12, pp. 1914-1920.
Tobin, Garry ; Yee, Douglas ; Brünner, Nils ; Rotwein, Peter. / A novel human insulin-like growth factor I messenger RNA is expressed in normal and tumor cells. In: Molecular Endocrinology. 1990 ; Vol. 4, No. 12. pp. 1914-1920.
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