A novel human cytomegalovirus locus modulates cell type-specific outcomes of infection

Mahadevaiah Umashankar, Alex Petrucelli, Louis Cicchini, Patrizia Caposio, Craig N. Kreklywich, Michael Rak, Farah Bughio, Devorah Goldman, Kimberly L. Hamlin, Jay Nelson, William Fleming, Daniel Streblow, Felicia Goodrum

Research output: Contribution to journalArticle

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Abstract

Clinical strains of HCMV encode 20 putative ORFs within a region of the genome termed ULb′ that are postulated to encode functions related to persistence or immune evasion. We have previously identified ULb′-encoded pUL138 as necessary, but not sufficient, for HCMV latency in CD34+ hematopoietic progenitor cells (HPCs) infected in vitro. pUL138 is encoded on polycistronic transcripts that also encode 3 additional proteins, pUL133, pUL135, and pUL136, collectively comprising the UL133-UL138 locus. This work represents the first characterization of these proteins and identifies a role for this locus in infection. Similar to pUL138, pUL133, pUL135, and pUL136 are integral membrane proteins that partially co-localized with pUL138 in the Golgi during productive infection in fibroblasts. As expected of ULb′ sequences, the UL133-UL138 locus was dispensable for replication in cultured fibroblasts. In CD34+ HPCs, this locus suppressed viral replication in HPCs, an activity attributable to both pUL133 and pUL138. Strikingly, the UL133-UL138 locus was required for efficient replication in endothelial cells. The association of this locus with three context-dependent phenotypes suggests an exciting role for the UL133-UL138 locus in modulating the outcome of viral infection in different contexts of infection. Differential profiles of protein expression from the UL133-UL138 locus correlated with the cell-type dependent phenotypes associated with this locus. We extended our in vitro findings to analyze viral replication and dissemination in a NOD-scid IL2Rγcnull-humanized mouse model. The UL133-UL138NULL virus exhibited an increased capacity for replication and/or dissemination following stem cell mobilization relative to the wild-type virus, suggesting an important role in viral persistence and spread in the host. As pUL133, pUL135, pUL136, and pUL138 are conserved in virus strains infecting higher order primates, but not lower order mammals, the functions encoded likely represent host-specific viral adaptations.

Original languageEnglish (US)
Article numbere1002444
JournalPLoS Pathogens
Volume7
Issue number12
DOIs
StatePublished - Dec 2011

Fingerprint

Hematopoietic Stem Cells
Cytomegalovirus
Viruses
Fibroblasts
Infection
Hematopoietic Stem Cell Mobilization
Phenotype
Immune Evasion
Proteins
Virus Diseases
Primates
Open Reading Frames
Mammals
Membrane Proteins
Endothelial Cells
Genome
In Vitro Techniques

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Umashankar, M., Petrucelli, A., Cicchini, L., Caposio, P., Kreklywich, C. N., Rak, M., ... Goodrum, F. (2011). A novel human cytomegalovirus locus modulates cell type-specific outcomes of infection. PLoS Pathogens, 7(12), [e1002444]. https://doi.org/10.1371/journal.ppat.1002444

A novel human cytomegalovirus locus modulates cell type-specific outcomes of infection. / Umashankar, Mahadevaiah; Petrucelli, Alex; Cicchini, Louis; Caposio, Patrizia; Kreklywich, Craig N.; Rak, Michael; Bughio, Farah; Goldman, Devorah; Hamlin, Kimberly L.; Nelson, Jay; Fleming, William; Streblow, Daniel; Goodrum, Felicia.

In: PLoS Pathogens, Vol. 7, No. 12, e1002444, 12.2011.

Research output: Contribution to journalArticle

Umashankar, M, Petrucelli, A, Cicchini, L, Caposio, P, Kreklywich, CN, Rak, M, Bughio, F, Goldman, D, Hamlin, KL, Nelson, J, Fleming, W, Streblow, D & Goodrum, F 2011, 'A novel human cytomegalovirus locus modulates cell type-specific outcomes of infection', PLoS Pathogens, vol. 7, no. 12, e1002444. https://doi.org/10.1371/journal.ppat.1002444
Umashankar, Mahadevaiah ; Petrucelli, Alex ; Cicchini, Louis ; Caposio, Patrizia ; Kreklywich, Craig N. ; Rak, Michael ; Bughio, Farah ; Goldman, Devorah ; Hamlin, Kimberly L. ; Nelson, Jay ; Fleming, William ; Streblow, Daniel ; Goodrum, Felicia. / A novel human cytomegalovirus locus modulates cell type-specific outcomes of infection. In: PLoS Pathogens. 2011 ; Vol. 7, No. 12.
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