A novel Arabidopsis type 1 protein phosphatase is highly expressed in male and female tissues and functionally complements a conditional cell cycle mutant of Aspergillus

A. Arundhati, Heidi Feiler, J. Traas, H. Zhang, P. A. Lunness, J. H. Doonan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Type 1 protein phosphatases are very highly conserved throughout eukaryotes where they regulate a number of key metabolic and morphogenetic processes. A cDNA, AtPP1bg, representing a new member of the type 1 protein phosphatase gene family in Arabidopsis has been isolated on the basis of hybridization with the Aspergillus bimG protein phosphatase gene. The AtPP1bg gene potentially encodes a 37 RDa protein very closely related to PP1 but with divergent N- and C-termini. The predicted amino acid sequence shows 71% identity to the ORF of the bimG gene. When expressed in Aspergillus under the alcA promoter, this phosphatase complements the temperature-sensitive bimG11 mutation allowing nearly normal vegetative growth at 37°C (but not at 42°C). Notably, the plant PP1 does not support morphogenesis (conidiation) at 37°C, This may indicate that conidophore formation has particular phosphatase requirement(s) which the plant PP1 cannot supply. The pattern of expression of the AtPP1bg transcript has been studied during development of the plant. In situ hybridization of Arabidopsis with antisense probes shows that this phosphatase gene is expressed at a low level throughout the plant, but is strongly upregulated within developing flowers, especially in the tapetum, the developing and mature pollen and in the ovaries. This implies that the AtPP1bg either has a specialized role in the formation of these organs, or that there is an increased requirement for protein phosphatase 1 at these stages. It was found that the level of AtPP1bg transcript, as judged by the relative intensity of staining in different cells within the floral meristems, did not vary during the cell cycle.

Original languageEnglish (US)
Pages (from-to)823-834
Number of pages12
JournalPlant Journal
Volume7
Issue number5
StatePublished - May 1995
Externally publishedYes

Fingerprint

Arabidopsis Proteins
phosphoprotein phosphatase
Phosphoprotein Phosphatases
Aspergillus
cell cycle
Cell Cycle
complement
Protein Phosphatase 1
Arabidopsis
mutants
Phosphoric Monoester Hydrolases
Genes
genes
Plant Development
Meristem
Pollen
Eukaryota
Morphogenesis
meristems
in situ hybridization

ASJC Scopus subject areas

  • Plant Science

Cite this

A novel Arabidopsis type 1 protein phosphatase is highly expressed in male and female tissues and functionally complements a conditional cell cycle mutant of Aspergillus. / Arundhati, A.; Feiler, Heidi; Traas, J.; Zhang, H.; Lunness, P. A.; Doonan, J. H.

In: Plant Journal, Vol. 7, No. 5, 05.1995, p. 823-834.

Research output: Contribution to journalArticle

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