A new model of cortical stroke in the rhesus macaque

G. Alexander West, Kiarash J. Golshani, Kristian P. Doyle, Nikola S. Lessov, Theodore R. Hobbs, Steven G. Kohama, Martin M. Pike, Christopher D. Kroenke, Marjorie R. Grafe, Maxwell D. Spector, Eric T. Tobar, Roger P. Simon, Mary P. Stenzel-Poore

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Primate models are essential tools for translational research in stroke but are reportedly inconsistent in their ability to produce cortical infarcts of reproducible size. Here, we report a new stroke model using a transorbital, reversible, two-vessel occlusion approach in male rhesus macaques that produces consistent and reproducible cortical infarcts. The right middle cerebral artery (distal to the orbitofrontal branch) and both anterior cerebral arteries were occluded with vascular clips. Bilateral occlusion of the anterior cerebral artery was critical for reducing collateral flow to the ipsilateral cortex. Reversible ischemia was induced for 45, 60, or 90 mins (n2/timepoint) and infarct volume and neurologic outcome were evaluated. The infarcts were located predominantly in the cortex and increased in size with extended duration of ischemia determined by T 2 -weighted magnetic resonance imaging. Infarct volume measured by 2,3,5-triphenyl tetrazolium chloride and cresyl violet staining corroborated magnetic resonance imaging results. Neurologic deficit scores worsened gradually with longer occlusion times. A subset of animals (n5) underwent 60 mins of ischemia resulting in consistent infarct volumes primarily located to the cortex that correlated well with neurologic deficit scores. This approach offers promise for evaluating therapeutic interventions in stroke.

Original languageEnglish (US)
Pages (from-to)1175-1186
Number of pages12
JournalJournal of Cerebral Blood Flow and Metabolism
Volume29
Issue number6
DOIs
StatePublished - Jun 2009

Keywords

  • Cerebral ischemia
  • MRI
  • Neurologic deficit
  • Nonhuman primate
  • Rhesus macaque
  • Stroke

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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