A new method for PCR products cloning - T-A cloning technique is introduced. pBluescript SK (+) plasmid was extracted by modified alkali lysis method. A blunt ends was generated using restriction enzyme EcoRV , then T-A cloning vector was prepared in the presence of Taq DNA polymerase and dTTP. β-actin cDNA fragment was cloned into the above T-A cloning vector. After the recombinant plasmids were digested by EcoR I and HindIII, an expected size of β-actin cDNA fragment was obtained.
|Original language||English (US)|
|Number of pages||1|
|Journal||Progress in Biochemistry and Biophysics|
|State||Published - 1999|
- PCR products
- T-A cloning vector
ASJC Scopus subject areas