Abstract
A new method for PCR products cloning - T-A cloning technique is introduced. pBluescript SK (+) plasmid was extracted by modified alkali lysis method. A blunt ends was generated using restriction enzyme EcoRV , then T-A cloning vector was prepared in the presence of Taq DNA polymerase and dTTP. β-actin cDNA fragment was cloned into the above T-A cloning vector. After the recombinant plasmids were digested by EcoR I and HindIII, an expected size of β-actin cDNA fragment was obtained.
Original language | English (US) |
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Pages (from-to) | 189 |
Number of pages | 1 |
Journal | Progress in Biochemistry and Biophysics |
Volume | 26 |
Issue number | 2 |
State | Published - 1999 |
Externally published | Yes |
Keywords
- PCR products
- T-A cloning vector
- β-actin
ASJC Scopus subject areas
- Biophysics
- Biochemistry