A new DNA polymerase activity of Escherichia coli. I. Purification and properties of the activity present in E. coli polAl

Robb Moses, Charles C. Richardson

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

A new DNA polymerase activity present in cells of Escherichia coli has been purified to homogeneity from E. coli polAl. The new enzyme (polymerase II) differs from the previously described polymerase (polymerase I) in chromatographic properties, sensitivity to sulfhydryl-blocking agents, response to antibody, and template requirements.

Original languageEnglish (US)
Pages (from-to)1557-1564
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume41
Issue number6
DOIs
StatePublished - Dec 24 1970
Externally publishedYes

Fingerprint

DNA-Directed DNA Polymerase
Escherichia coli
Purification
Antibody Formation
Antibodies
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A new DNA polymerase activity of Escherichia coli. I. Purification and properties of the activity present in E. coli polAl. / Moses, Robb; Richardson, Charles C.

In: Biochemical and Biophysical Research Communications, Vol. 41, No. 6, 24.12.1970, p. 1557-1564.

Research output: Contribution to journalArticle

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