A mutation in the 3-phosphoglycerate kinase gene allows anaerobic growth of Bacillus subtilis in the absence of ResE kinase

Michiko Nakano, Yi Zhu, Koki Haga, Hirofumi Yoshikawa, Abraham L. Sonenshein, Peter Zuber

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Abstract

The Bacillus subtilis ResD-ResE two-component signal transduction system is essential for aerobic and anaerobic respiration. A spontaneous suppressor mutant that expresses ResD-controlled genes and grows anaerobically in the absence of the ResE histidine kinase was isolated. In addition, aerobic expression of ResD-controlled genes in the suppressed strain was constitutive and occurred at a much higher level than that observed in the wild-type strain. The suppressing mutation, which mapped to pgk, the gene encoding 3- phosphoglycerate kinase, failed to suppress a resD mutation, suggesting that the suppressing mutation creates a pathway for phosphorylation of the response regulator, ResD, which is independent of the cognate sensor kinase, ResE. The pgk-1 mutant exhibited very low but measurable 3-phosphoglycerate kinase activity compared to the wild-type strain. The results suggest that accumulation of a glycolytic intermediate, probably 1,3-diphosphoglycerate, is responsible for the observed effect of the pgk-1 mutation on anaerobiosis of resE mutant cells.

Original languageEnglish (US)
Pages (from-to)7087-7097
Number of pages11
JournalJournal of Bacteriology
Volume181
Issue number22
StatePublished - Nov 1999

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Phosphoglycerate Kinase
Bacillus subtilis
Phosphotransferases
Mutation
Growth
Genes
Anaerobiosis
Signal Transduction
Respiration
Phosphorylation
3-phosphoglycerate

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

A mutation in the 3-phosphoglycerate kinase gene allows anaerobic growth of Bacillus subtilis in the absence of ResE kinase. / Nakano, Michiko; Zhu, Yi; Haga, Koki; Yoshikawa, Hirofumi; Sonenshein, Abraham L.; Zuber, Peter.

In: Journal of Bacteriology, Vol. 181, No. 22, 11.1999, p. 7087-7097.

Research output: Contribution to journalArticle

Nakano, Michiko ; Zhu, Yi ; Haga, Koki ; Yoshikawa, Hirofumi ; Sonenshein, Abraham L. ; Zuber, Peter. / A mutation in the 3-phosphoglycerate kinase gene allows anaerobic growth of Bacillus subtilis in the absence of ResE kinase. In: Journal of Bacteriology. 1999 ; Vol. 181, No. 22. pp. 7087-7097.
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