A mutation in the 3-phosphoglycerate kinase gene allows anaerobic growth of Bacillus subtilis in the absence of ResE kinase

Michiko M. Nakano, Yi Zhu, Koki Haga, Hirofumi Yoshikawa, Abraham L. Sonenshein, Peter Zuber

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The Bacillus subtilis ResD-ResE two-component signal transduction system is essential for aerobic and anaerobic respiration. A spontaneous suppressor mutant that expresses ResD-controlled genes and grows anaerobically in the absence of the ResE histidine kinase was isolated. In addition, aerobic expression of ResD-controlled genes in the suppressed strain was constitutive and occurred at a much higher level than that observed in the wild-type strain. The suppressing mutation, which mapped to pgk, the gene encoding 3- phosphoglycerate kinase, failed to suppress a resD mutation, suggesting that the suppressing mutation creates a pathway for phosphorylation of the response regulator, ResD, which is independent of the cognate sensor kinase, ResE. The pgk-1 mutant exhibited very low but measurable 3-phosphoglycerate kinase activity compared to the wild-type strain. The results suggest that accumulation of a glycolytic intermediate, probably 1,3-diphosphoglycerate, is responsible for the observed effect of the pgk-1 mutation on anaerobiosis of resE mutant cells.

Original languageEnglish (US)
Pages (from-to)7087-7097
Number of pages11
JournalJournal of bacteriology
Volume181
Issue number22
StatePublished - Nov 1 1999

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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