A multifunctional aqueous channel formed by CFTR

Hajime Hasegawa, William Skach, Oliver Baker, M. Clara Calayag, V. Lingappa, A. S. Verkman

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The cystic fibrosis gene product (CFTR) is a complex protein that functions as an adenosine 3,5-monophosphate (cAMP)-stimulated ion channel and possibly as a regulator of intracellular processes. In order to determine whether the CFTR molecule contains a functional aqueous pathway, anion, water, and urea transport were measured in Xenopus oocytes expressing CFTR. Cyclic AMP agonists induced a Cl- conductance of 94 microsiemens and an increase in water permeability of 4 × 10-4 centimeter per second that was inhibited by a Cl- channel blocker and was dependent on anion composition. CFTR has a calculated single channel water conductance of 9 × 10-13 cubic centimeter per second, suggesting a pore-like aqueous pathway. Oocytes expressing CFTR also showed cAMP-stimulated transport of urea but not the larger solute sucrose. Thus CFTR contains a cAMP-stimulated aqueous pore that can transport anions, water, and small solutes. The results also provide functional evidence for water movement through an ion channel.

Original languageEnglish (US)
Pages (from-to)1477-1479
Number of pages3
JournalScience
Volume258
Issue number5087
StatePublished - Nov 27 1992
Externally publishedYes

Fingerprint

Anions
Ion Channels
Oocytes
Urea
Water
Water Movements
Aquaporins
Xenopus
Cystic Fibrosis
Cyclic AMP
Adenosine
Sucrose
Permeability
Genes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Hasegawa, H., Skach, W., Baker, O., Calayag, M. C., Lingappa, V., & Verkman, A. S. (1992). A multifunctional aqueous channel formed by CFTR. Science, 258(5087), 1477-1479.

A multifunctional aqueous channel formed by CFTR. / Hasegawa, Hajime; Skach, William; Baker, Oliver; Calayag, M. Clara; Lingappa, V.; Verkman, A. S.

In: Science, Vol. 258, No. 5087, 27.11.1992, p. 1477-1479.

Research output: Contribution to journalArticle

Hasegawa, H, Skach, W, Baker, O, Calayag, MC, Lingappa, V & Verkman, AS 1992, 'A multifunctional aqueous channel formed by CFTR', Science, vol. 258, no. 5087, pp. 1477-1479.
Hasegawa H, Skach W, Baker O, Calayag MC, Lingappa V, Verkman AS. A multifunctional aqueous channel formed by CFTR. Science. 1992 Nov 27;258(5087):1477-1479.
Hasegawa, Hajime ; Skach, William ; Baker, Oliver ; Calayag, M. Clara ; Lingappa, V. ; Verkman, A. S. / A multifunctional aqueous channel formed by CFTR. In: Science. 1992 ; Vol. 258, No. 5087. pp. 1477-1479.
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