A multifunctional aqueous channel formed by CFTR

Hajime Hasegawa, William Skach, Oliver Baker, M. Clara Calayag, V. Lingappa, A. S. Verkman

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The cystic fibrosis gene product (CFTR) is a complex protein that functions as an adenosine 3,5-monophosphate (cAMP)-stimulated ion channel and possibly as a regulator of intracellular processes. In order to determine whether the CFTR molecule contains a functional aqueous pathway, anion, water, and urea transport were measured in Xenopus oocytes expressing CFTR. Cyclic AMP agonists induced a Cl- conductance of 94 microsiemens and an increase in water permeability of 4 × 10-4 centimeter per second that was inhibited by a Cl- channel blocker and was dependent on anion composition. CFTR has a calculated single channel water conductance of 9 × 10-13 cubic centimeter per second, suggesting a pore-like aqueous pathway. Oocytes expressing CFTR also showed cAMP-stimulated transport of urea but not the larger solute sucrose. Thus CFTR contains a cAMP-stimulated aqueous pore that can transport anions, water, and small solutes. The results also provide functional evidence for water movement through an ion channel.

Original languageEnglish (US)
Pages (from-to)1477-1479
Number of pages3
Issue number5087
StatePublished - Jan 1 1992

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    Hasegawa, H., Skach, W., Baker, O., Calayag, M. C., Lingappa, V., & Verkman, A. S. (1992). A multifunctional aqueous channel formed by CFTR. Science, 258(5087), 1477-1479. https://doi.org/10.1126/science.1279809