A multifunctional aqueous channel formed by CFTR

Hajime Hasegawa, William Skach, Oliver Baker, M. Clara Calayag, V. Lingappa, A. S. Verkman

Research output: Contribution to journalArticlepeer-review

121 Scopus citations

Abstract

The cystic fibrosis gene product (CFTR) is a complex protein that functions as an adenosine 3,5-monophosphate (cAMP)-stimulated ion channel and possibly as a regulator of intracellular processes. In order to determine whether the CFTR molecule contains a functional aqueous pathway, anion, water, and urea transport were measured in Xenopus oocytes expressing CFTR. Cyclic AMP agonists induced a Cl- conductance of 94 microsiemens and an increase in water permeability of 4 × 10-4 centimeter per second that was inhibited by a Cl- channel blocker and was dependent on anion composition. CFTR has a calculated single channel water conductance of 9 × 10-13 cubic centimeter per second, suggesting a pore-like aqueous pathway. Oocytes expressing CFTR also showed cAMP-stimulated transport of urea but not the larger solute sucrose. Thus CFTR contains a cAMP-stimulated aqueous pore that can transport anions, water, and small solutes. The results also provide functional evidence for water movement through an ion channel.

Original languageEnglish (US)
Pages (from-to)1477-1479
Number of pages3
JournalScience
Volume258
Issue number5087
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • General

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