A mouse model of episodic ataxia type-1

Paco S. Herson, Michael Virk, Nathan R. Rustay, Chris T. Bond, John Jr Crabbe, John Adelman, James Maylie

Research output: Contribution to journalArticle

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Abstract

Episodic ataxia type-1 (EA1) is a dominant human neurological disorder characterized by stress-induced attacks of ataxia. EA1 is caused by mutations in the voltage-gated potassium channel Kv1.1, and affected individuals are heterozygous. Here we introduced the V408A EA1 mutation into mice using homologous recombination. In contrast to Kv1.1 null mice, homozygous V408A/V408A mice died after embryonic day 3 (E3). V408A/+ mice showed stress-induced loss of motor coordination that was ameliorated by acetazolamide, a carbonic anhydrase inhibitor that minimizes EA1 symptoms in human patients. We made electrophysiological recordings from cerebellar Purkinje cells in both V408A/+ mice and their wild-type littermates. V408A/+ mice showed a greater frequency and amplitude of spontaneous GABAergic inhibitory postsynaptic currents (IPSCs) than did wild type; however, the amplitude or frequency of miniature IPSCs and the basket cell firing frequency did not differ between groups. The stress-induced motor dysfunction in V408A mice is similar to that of family members harboring the EA1 allele, and our findings suggest that these behavioral changes are linked to changes in GABA release.

Original languageEnglish (US)
Pages (from-to)378-383
Number of pages6
JournalNature Neuroscience
Volume6
Issue number4
DOIs
StatePublished - Apr 1 2003

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Inhibitory Postsynaptic Potentials
Carbonic Anhydrase Inhibitors
Voltage-Gated Potassium Channels
Mutation
Acetazolamide
Purkinje Cells
Homologous Recombination
Ataxia
Type 1 Episodic Ataxia
Nervous System Diseases
gamma-Aminobutyric Acid
Alleles

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Herson, P. S., Virk, M., Rustay, N. R., Bond, C. T., Crabbe, J. J., Adelman, J., & Maylie, J. (2003). A mouse model of episodic ataxia type-1. Nature Neuroscience, 6(4), 378-383. https://doi.org/10.1038/nn1025

A mouse model of episodic ataxia type-1. / Herson, Paco S.; Virk, Michael; Rustay, Nathan R.; Bond, Chris T.; Crabbe, John Jr; Adelman, John; Maylie, James.

In: Nature Neuroscience, Vol. 6, No. 4, 01.04.2003, p. 378-383.

Research output: Contribution to journalArticle

Herson, PS, Virk, M, Rustay, NR, Bond, CT, Crabbe, JJ, Adelman, J & Maylie, J 2003, 'A mouse model of episodic ataxia type-1', Nature Neuroscience, vol. 6, no. 4, pp. 378-383. https://doi.org/10.1038/nn1025
Herson PS, Virk M, Rustay NR, Bond CT, Crabbe JJ, Adelman J et al. A mouse model of episodic ataxia type-1. Nature Neuroscience. 2003 Apr 1;6(4):378-383. https://doi.org/10.1038/nn1025
Herson, Paco S. ; Virk, Michael ; Rustay, Nathan R. ; Bond, Chris T. ; Crabbe, John Jr ; Adelman, John ; Maylie, James. / A mouse model of episodic ataxia type-1. In: Nature Neuroscience. 2003 ; Vol. 6, No. 4. pp. 378-383.
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