A mouse model of elevated intraocular pressure: Retina and optic nerve findings

Ronald L. Gross, Jianzhong Ji, Peter Chang, Mark Pennesi, Zhuo Yang, Jian Zhang, Samuel M. Wu, Paul R. Lichter, George L. Spaeth, John C. Heckenlively, E. Michael Van Buskirk, David L. Knox

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Purpose: To develop and characterize a mouse model of elevated intraocular pressure (IOP) as a means to investigate the underlying cellular and genetic mechanisms of glaucomatous optic neuropathy. Methods: An experimental increase in IOP was induced in one eye of each adult C57BL/6J mouse by argon laser photocoagulation of the episcleral and limbal veins. The IOP of both eyes of each mouse was measured using an indentation tonometer prior to treatment and once a week thereafter. The mouse retinal ganglion cells (RGCs) were identified immunocytochemically using an antiserum against Thy1,2, CD90.2, and the number of RGCs was measured with confocal microscopy. The reduction in the number of RGCs was compared in the experimental and control eyes. The mechanism of RGC death after IOP elevation was investigated using TdT-mediated dUTP nick end labeling (TUNEL) staining. The pathologic changes of optic nerve following elevated IOP were characterized by light and electron microscopy. Results: After laser treatment, mean IOP was increased in the treated eyes from the control mean of 13 ± 1.8 mm Hg to 20.0 ± 2.8 mm Hg at 4 weeks. Peak IOP was 32 ± 2.5 mm Hg in the experimental group. RGC loss was 16. 9% ± 7.8% at 2 weeks (n = 6, P

Original languageEnglish (US)
Pages (from-to)163-171
Number of pages9
JournalTransactions of the American Ophthalmological Society
Volume101
StatePublished - 2003
Externally publishedYes

Fingerprint

Optic Nerve
Intraocular Pressure
Retina
Retinal Ganglion Cells
Lasers
Optic Nerve Diseases
Light Coagulation
Argon
Inbred C57BL Mouse
Confocal Microscopy
Immune Sera
Veins
Electron Microscopy
Cell Death
Staining and Labeling
Light
Therapeutics

ASJC Scopus subject areas

  • Ophthalmology

Cite this

A mouse model of elevated intraocular pressure : Retina and optic nerve findings. / Gross, Ronald L.; Ji, Jianzhong; Chang, Peter; Pennesi, Mark; Yang, Zhuo; Zhang, Jian; Wu, Samuel M.; Lichter, Paul R.; Spaeth, George L.; Heckenlively, John C.; Van Buskirk, E. Michael; Knox, David L.

In: Transactions of the American Ophthalmological Society, Vol. 101, 2003, p. 163-171.

Research output: Contribution to journalArticle

Gross, RL, Ji, J, Chang, P, Pennesi, M, Yang, Z, Zhang, J, Wu, SM, Lichter, PR, Spaeth, GL, Heckenlively, JC, Van Buskirk, EM & Knox, DL 2003, 'A mouse model of elevated intraocular pressure: Retina and optic nerve findings', Transactions of the American Ophthalmological Society, vol. 101, pp. 163-171.
Gross, Ronald L. ; Ji, Jianzhong ; Chang, Peter ; Pennesi, Mark ; Yang, Zhuo ; Zhang, Jian ; Wu, Samuel M. ; Lichter, Paul R. ; Spaeth, George L. ; Heckenlively, John C. ; Van Buskirk, E. Michael ; Knox, David L. / A mouse model of elevated intraocular pressure : Retina and optic nerve findings. In: Transactions of the American Ophthalmological Society. 2003 ; Vol. 101. pp. 163-171.
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AU - Ji, Jianzhong

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AU - Pennesi, Mark

AU - Yang, Zhuo

AU - Zhang, Jian

AU - Wu, Samuel M.

AU - Lichter, Paul R.

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AU - Van Buskirk, E. Michael

AU - Knox, David L.

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