A monospecific antibody to human sulfatase a preparation, characterization and significance

E. Neuwelt, D. Stumpf, J. Austin, P. Kohler

Research output: Contribution to journalArticle

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Abstract

A human enzyme, sulfatase A (arylsulfatase A, EC 3.1.6.1), was fractionated from liver and purified by electrofocusing. When injected into rabbits, the enzyme served as an antigen and induced the formation of antibodies. A monospecific antibody was then obtained when this rabbit antiserum was absorbed with human liver fractions which had been isoelectrically focused next to sulfatase A. Reaction kinetics between this anti-sulfatase A and sulfatases A and B were then studied. The activity of the enzyme was enhanced by the antibody under specified conditions. Thereafter, the antibody could be used to purify the enzyme relatively simply and rapidly.

Original languageEnglish (US)
Pages (from-to)333-346
Number of pages14
JournalBBA - Protein Structure
Volume236
Issue number1
DOIs
StatePublished - Apr 27 1971

ASJC Scopus subject areas

  • Medicine(all)

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