TY - JOUR
T1 - A monospecific antibody to human sulfatase a preparation, characterization and significance
AU - Neuwelt, E.
AU - Stumpf, D.
AU - Austin, J.
AU - Kohler, P.
N1 - Funding Information:
This study was supported by U.S. Public Health Service Grants No. NB-o77 I, STS-GH-I662-o5, and HD 03381-03, and in part by National Institutes of Health Research Grant No. FR-oo4o4-o2 from the Division of Research Facilities and Resources.
PY - 1971/4/27
Y1 - 1971/4/27
N2 - A human enzyme, sulfatase A (arylsulfatase A, EC 3.1.6.1), was fractionated from liver and purified by electrofocusing. When injected into rabbits, the enzyme served as an antigen and induced the formation of antibodies. A monospecific antibody was then obtained when this rabbit antiserum was absorbed with human liver fractions which had been isoelectrically focused next to sulfatase A. Reaction kinetics between this anti-sulfatase A and sulfatases A and B were then studied. The activity of the enzyme was enhanced by the antibody under specified conditions. Thereafter, the antibody could be used to purify the enzyme relatively simply and rapidly.
AB - A human enzyme, sulfatase A (arylsulfatase A, EC 3.1.6.1), was fractionated from liver and purified by electrofocusing. When injected into rabbits, the enzyme served as an antigen and induced the formation of antibodies. A monospecific antibody was then obtained when this rabbit antiserum was absorbed with human liver fractions which had been isoelectrically focused next to sulfatase A. Reaction kinetics between this anti-sulfatase A and sulfatases A and B were then studied. The activity of the enzyme was enhanced by the antibody under specified conditions. Thereafter, the antibody could be used to purify the enzyme relatively simply and rapidly.
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U2 - 10.1016/0005-2795(71)90182-6
DO - 10.1016/0005-2795(71)90182-6
M3 - Article
C2 - 4996027
AN - SCOPUS:0015242019
SN - 0005-2795
VL - 236
SP - 333
EP - 346
JO - BBA - Protein Structure
JF - BBA - Protein Structure
IS - 1
ER -