A human enzyme, sulfatase A (arylsulfatase A, EC 220.127.116.11), was fractionated from liver and purified by electrofocusing. When injected into rabbits, the enzyme served as an antigen and induced the formation of antibodies. A monospecific antibody was then obtained when this rabbit antiserum was absorbed with human liver fractions which had been isoelectrically focused next to sulfatase A. Reaction kinetics between this anti-sulfatase A and sulfatases A and B were then studied. The activity of the enzyme was enhanced by the antibody under specified conditions. Thereafter, the antibody could be used to purify the enzyme relatively simply and rapidly.
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