A monokine regulates colony-stimulating activity production by vascular endothelial cells

G. C. Bagby, E. McCall, K. A. Bergstrom, D. Burger

    Research output: Contribution to journalArticle

    51 Scopus citations

    Abstract

    Human umbilical vein endothelial cells were cultured in supernatants of peripheral blood monocytes that had been cultured for 3 days with and without lactoferrin. Colony-stimulating activity (CSA) was measured in supernatants of the endothelial cell cultures and appropriate control cultures using normal T-lymphocyte-depleted, phagocyte-depleted, low-density bone marrow cells in colony growth (CFU-GM) assays. Monocyte-conditioned medium contained a nondialyzable, heat labile factor that enhanced 4-15 fold the production of CSA by endothelial cells. The addition of lactoferrin to monocyte cultures reduced the activity of this monokine by 69%. Lactoferrin did not inhibit CSA production by monokine-stimulated endothelial cells. Therefore, the production of CSA by these cells is regulated by a stimulatory monokine, and the production and/or release of the monokinase is inhibited by lactoferrin, a neutrophil-derived putative feedback inhibitor of granulopoiesis. Inasmuch as a similar monokine is known to stimulate CSA production by fibroblasts and T lymphocytes, we suggest that mononuclear phagocytes play a pivotal role in the regulation of granulopoiesis by recruiting a variety of cell types to produce CSA.

    Original languageEnglish (US)
    Pages (from-to)663-668
    Number of pages6
    JournalBlood
    Volume62
    Issue number3
    DOIs
    StatePublished - Jan 1 1983

    ASJC Scopus subject areas

    • Biochemistry
    • Immunology
    • Hematology
    • Cell Biology

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