A monokine regulates colony-stimulating activity production by vascular endothelial cells

G. C. Bagby, E. McCall, K. A. Bergstrom, D. Burger

    Research output: Contribution to journalArticle

    51 Citations (Scopus)

    Abstract

    Human umbilical vein endothelial cells were cultured in supernatants of peripheral blood monocytes that had been cultured for 3 days with and without lactoferrin. Colony-stimulating activity (CSA) was measured in supernatants of the endothelial cell cultures and appropriate control cultures using normal T-lymphocyte-depleted, phagocyte-depleted, low-density bone marrow cells in colony growth (CFU-GM) assays. Monocyte-conditioned medium contained a nondialyzable, heat labile factor that enhanced 4-15 fold the production of CSA by endothelial cells. The addition of lactoferrin to monocyte cultures reduced the activity of this monokine by 69%. Lactoferrin did not inhibit CSA production by monokine-stimulated endothelial cells. Therefore, the production of CSA by these cells is regulated by a stimulatory monokine, and the production and/or release of the monokinase is inhibited by lactoferrin, a neutrophil-derived putative feedback inhibitor of granulopoiesis. Inasmuch as a similar monokine is known to stimulate CSA production by fibroblasts and T lymphocytes, we suggest that mononuclear phagocytes play a pivotal role in the regulation of granulopoiesis by recruiting a variety of cell types to produce CSA.

    Original languageEnglish (US)
    Pages (from-to)663-668
    Number of pages6
    JournalBlood
    Volume62
    Issue number3
    StatePublished - 1983

    Fingerprint

    Monokines
    Lactoferrin
    Endothelial cells
    Endothelial Cells
    Monocytes
    Cell culture
    Phagocytes
    T-cells
    T-Lymphocytes
    Granulocyte-Macrophage Progenitor Cells
    Human Umbilical Vein Endothelial Cells
    Conditioned Culture Medium
    Bone Marrow Cells
    Fibroblasts
    Neutrophils
    Cell Culture Techniques
    Hot Temperature
    Assays
    Bone
    Blood

    ASJC Scopus subject areas

    • Hematology

    Cite this

    Bagby, G. C., McCall, E., Bergstrom, K. A., & Burger, D. (1983). A monokine regulates colony-stimulating activity production by vascular endothelial cells. Blood, 62(3), 663-668.

    A monokine regulates colony-stimulating activity production by vascular endothelial cells. / Bagby, G. C.; McCall, E.; Bergstrom, K. A.; Burger, D.

    In: Blood, Vol. 62, No. 3, 1983, p. 663-668.

    Research output: Contribution to journalArticle

    Bagby, GC, McCall, E, Bergstrom, KA & Burger, D 1983, 'A monokine regulates colony-stimulating activity production by vascular endothelial cells', Blood, vol. 62, no. 3, pp. 663-668.
    Bagby, G. C. ; McCall, E. ; Bergstrom, K. A. ; Burger, D. / A monokine regulates colony-stimulating activity production by vascular endothelial cells. In: Blood. 1983 ; Vol. 62, No. 3. pp. 663-668.
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    abstract = "Human umbilical vein endothelial cells were cultured in supernatants of peripheral blood monocytes that had been cultured for 3 days with and without lactoferrin. Colony-stimulating activity (CSA) was measured in supernatants of the endothelial cell cultures and appropriate control cultures using normal T-lymphocyte-depleted, phagocyte-depleted, low-density bone marrow cells in colony growth (CFU-GM) assays. Monocyte-conditioned medium contained a nondialyzable, heat labile factor that enhanced 4-15 fold the production of CSA by endothelial cells. The addition of lactoferrin to monocyte cultures reduced the activity of this monokine by 69{\%}. Lactoferrin did not inhibit CSA production by monokine-stimulated endothelial cells. Therefore, the production of CSA by these cells is regulated by a stimulatory monokine, and the production and/or release of the monokinase is inhibited by lactoferrin, a neutrophil-derived putative feedback inhibitor of granulopoiesis. Inasmuch as a similar monokine is known to stimulate CSA production by fibroblasts and T lymphocytes, we suggest that mononuclear phagocytes play a pivotal role in the regulation of granulopoiesis by recruiting a variety of cell types to produce CSA.",
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