A mitogen for human B cells: Anti-Ig coupled to polyacrylamide beads activates blood mononuclear cells independently of T cells

J. J. Fothergill, R. Wistar, J. N. Woody, David Parker

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The F(ab')2 fragment of rabbit anti-human F(ab')2 antibodies covalently linked to polyacrylamide beads (anti-Ig beads) acts as a polyclonal mitogen for human B cells in whole peripheral blood mononuclear cells (PBM) or tonsil cells. Activation, measured by the rate of DNA synthesis, is the result of an interaction between the anti-Ig beads and membrane immunoglobulin found on B cells. Excess soluble human F(ab')2 blocks activation with anti-Ig beads. Immunospecifically purified antibodies against IgM or IgD bound to beads activate lymphocytes. Also soluble anti-IgM or anti-IgD can block activation with insolubilized anti-IgM or anti-IgD, respectively, by modulating membrane immunoglobulin. Irrelevant rabbit anti-hapten antibodies bound to beads failed to activate PBM or tonsil cells. Anti-Ig beads act independently of T cells. The anti-Ig beads lead to activation of B cells without further maturation to polyclonal immunoglobulin secretion.

Original languageEnglish (US)
Pages (from-to)1945-1949
Number of pages5
JournalJournal of Immunology
Volume128
Issue number5
StatePublished - 1982
Externally publishedYes

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Mitogens
Immunoglobulins
Blood Cells
B-Lymphocytes
Palatine Tonsil
T-Lymphocytes
Rabbits
Immunoglobulin D
Membranes
Antibodies
Haptens
Immunoglobulin M
Anti-Idiotypic Antibodies
Lymphocytes
DNA
polyacrylamide
anti-IgM
anti-IgD

ASJC Scopus subject areas

  • Immunology

Cite this

A mitogen for human B cells : Anti-Ig coupled to polyacrylamide beads activates blood mononuclear cells independently of T cells. / Fothergill, J. J.; Wistar, R.; Woody, J. N.; Parker, David.

In: Journal of Immunology, Vol. 128, No. 5, 1982, p. 1945-1949.

Research output: Contribution to journalArticle

Fothergill, J. J. ; Wistar, R. ; Woody, J. N. ; Parker, David. / A mitogen for human B cells : Anti-Ig coupled to polyacrylamide beads activates blood mononuclear cells independently of T cells. In: Journal of Immunology. 1982 ; Vol. 128, No. 5. pp. 1945-1949.
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