A mitogen for human B cells: Anti-Ig coupled to polyacrylamide beads activates blood mononuclear cells independently of T cells

J. J. Fothergill, R. Wistar, J. N. Woody, D. C. Parker

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The F(ab')2 fragment of rabbit anti-human F(ab')2 antibodies covalently linked to polyacrylamide beads (anti-Ig beads) acts as a polyclonal mitogen for human B cells in whole peripheral blood mononuclear cells (PBM) or tonsil cells. Activation, measured by the rate of DNA synthesis, is the result of an interaction between the anti-Ig beads and membrane immunoglobulin found on B cells. Excess soluble human F(ab')2 blocks activation with anti-Ig beads. Immunospecifically purified antibodies against IgM or IgD bound to beads activate lymphocytes. Also soluble anti-IgM or anti-IgD can block activation with insolubilized anti-IgM or anti-IgD, respectively, by modulating membrane immunoglobulin. Irrelevant rabbit anti-hapten antibodies bound to beads failed to activate PBM or tonsil cells. Anti-Ig beads act independently of T cells. The anti-Ig beads lead to activation of B cells without further maturation to polyclonal immunoglobulin secretion.

Original languageEnglish (US)
Pages (from-to)1945-1949
Number of pages5
JournalJournal of Immunology
Volume128
Issue number5
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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