A maternal high-fat diet modulates fetal SIRT1 histone and protein deacetylase activity in nonhuman primates

Melissa A. Suter, Aishe Chen, Marie S. Burdine, Mahua Choudhury, R. Alan Harris, Robert H. Lane, Jacob E. Friedman, Kevin Grove, Alan J. Tackett, Kjersti M. Aagaard

    Research output: Contribution to journalArticle

    84 Citations (Scopus)

    Abstract

    In nonhuman primates, we previously demonstrated that a maternal high-fat diet (MHFD) induces fetal nonalcoholic fatty liver disease (NAFLD) and alters the fetal metabolome. These changes are accompanied by altered acetylation of histone H3 (H3K14ac). However, the mechanism behind this alteration in acetylation remains unknown. As SIRT1 is both a lysine deacetylase and a crucial sensor of cellular metabolism, we hypothesized that SIRT1 may be involved in fetal epigenomic alterations. Here we show that in utero exposure to a MHFD, but not maternal obesity per se, increases fetal H3K14ac with concomitant decreased SIRT1 expression and diminished in vitro protein and histone deacetylase activity. MHFD increased H3K14ac and DBC1-SIRT1 complex formation in fetal livers, both of which were abrogated with diet reversal despite persistent maternal obesity. Moreover, MHFD was associated with altered expression of known downstream effectors deregulated in NAFLD and modulated by SIRT1 (e.g., PPARA, PPARG, SREBF1, CYP7A1, FASN, and SCD). Finally, ex vivo purified SIRT1 retains deacetylase activity on an H3K14ac peptide substrate with preferential activity toward acetylated histone H3; mutagenesis of the catalytic domain of SIRT1 (H363Y) abrogates H3K14ac deacetylation. Our data implicate SIRT1 as a likely molecular mediator of the fetal epigenome and metabolome under MHFD conditions.

    Original languageEnglish (US)
    Pages (from-to)5106-5114
    Number of pages9
    JournalFASEB Journal
    Volume26
    Issue number12
    DOIs
    StatePublished - Dec 2012

    Fingerprint

    Histone Deacetylases
    High Fat Diet
    Nutrition
    Histones
    Primates
    Fats
    Mothers
    Liver
    Acetylation
    Proteins
    Metabolome
    Obesity
    Mutagenesis
    Metabolism
    Lysine
    Epigenomics
    Catalytic Domain
    Peptides
    Diet
    Sensors

    Keywords

    • Developmental origins of adult disease
    • Epigenetics
    • Histone modification
    • NAFLD
    • Sirtuins

    ASJC Scopus subject areas

    • Biochemistry
    • Biotechnology
    • Genetics
    • Molecular Biology

    Cite this

    Suter, M. A., Chen, A., Burdine, M. S., Choudhury, M., Harris, R. A., Lane, R. H., ... Aagaard, K. M. (2012). A maternal high-fat diet modulates fetal SIRT1 histone and protein deacetylase activity in nonhuman primates. FASEB Journal, 26(12), 5106-5114. https://doi.org/10.1096/fj.12-212878

    A maternal high-fat diet modulates fetal SIRT1 histone and protein deacetylase activity in nonhuman primates. / Suter, Melissa A.; Chen, Aishe; Burdine, Marie S.; Choudhury, Mahua; Harris, R. Alan; Lane, Robert H.; Friedman, Jacob E.; Grove, Kevin; Tackett, Alan J.; Aagaard, Kjersti M.

    In: FASEB Journal, Vol. 26, No. 12, 12.2012, p. 5106-5114.

    Research output: Contribution to journalArticle

    Suter, MA, Chen, A, Burdine, MS, Choudhury, M, Harris, RA, Lane, RH, Friedman, JE, Grove, K, Tackett, AJ & Aagaard, KM 2012, 'A maternal high-fat diet modulates fetal SIRT1 histone and protein deacetylase activity in nonhuman primates', FASEB Journal, vol. 26, no. 12, pp. 5106-5114. https://doi.org/10.1096/fj.12-212878
    Suter, Melissa A. ; Chen, Aishe ; Burdine, Marie S. ; Choudhury, Mahua ; Harris, R. Alan ; Lane, Robert H. ; Friedman, Jacob E. ; Grove, Kevin ; Tackett, Alan J. ; Aagaard, Kjersti M. / A maternal high-fat diet modulates fetal SIRT1 histone and protein deacetylase activity in nonhuman primates. In: FASEB Journal. 2012 ; Vol. 26, No. 12. pp. 5106-5114.
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