A mammalian Dna- mutant decreasing nuclear DNA polymerase α activity at nonpermissive temperature

Yan Jyu Tsai, Fumio Hanaoka, Michiko M. Nakano, Masa atsu Yamada

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21 Scopus citations


A temperature-sensitive Dna- mutant (ts T244) of the mouse FM3A cell line was characterized. ts T244 showed no increase in cell number and rapid decrease in the rate of DNA synthesis after temperature upshift (33°C→39°C). The activity of nuclear DNA polymerase α decreased as rapidly as the rate of DNA synthesis, whereas the activities of thymidine kinase and thymidylate kinase decreased more slowly. The results suggest that the level of nuclear DNA polymerase α has close relation to the ts defect of this Dna- mutant.

Original languageEnglish (US)
Pages (from-to)1190-1195
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Dec 14 1979


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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