Abstract
We have isolated a mutant clone from mouse FM3A cells with temperature-sensitive defects both in cytokinesis and in thymidine kinase enzyme activity. The clone, designated tsCl.B59, was isolated after mutagenesis at 33°C followed by exposure to cytosine arabinoside at 39°C. It was derived from a thymidine kinase deficient, 5-bromodeoxyuridine-resistant clone (S-BUCl.42) which was originally derived from wild-type clone H-5 of FM3A cells. The temperature-sensitive mutant clone grows normally at 33°C, but not at 39°C, where it exhibits an increased frequency of multinucleate cells due to defective cytokinesis. Unlike the parental S-BUCl.42 cells, which have negligible thymidine kinase activity and are unable to incorporate3H-thymidine, the mutant incorporates substantial amounts of3H-thymidine at 33°C, although its thymidine kinase activity remains lower than that of wild-type H-5 cells. When cultures of tsCl.B59 cells are transferred to 39°C, incorporation of3H-thymidine decreases markedly. The decrease has been shown to be due to thermolability of the thymidine kinase in tsCl.B59 cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 169-178 |
| Number of pages | 10 |
| Journal | Somatic Cell Genetics |
| Volume | 4 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 1978 |
| Externally published | Yes |
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- Genetics
- Medicine(all)
Cite this
A mammalian cell mutant with temperature-sensitive thymidine kinase. / Nakano, Michiko; Sekiguchi, Toyozo; Yamada, Masa atsu.
In: Somatic Cell Genetics, Vol. 4, No. 2, 03.1978, p. 169-178.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A mammalian cell mutant with temperature-sensitive thymidine kinase
AU - Nakano, Michiko
AU - Sekiguchi, Toyozo
AU - Yamada, Masa atsu
PY - 1978/3
Y1 - 1978/3
N2 - We have isolated a mutant clone from mouse FM3A cells with temperature-sensitive defects both in cytokinesis and in thymidine kinase enzyme activity. The clone, designated tsCl.B59, was isolated after mutagenesis at 33°C followed by exposure to cytosine arabinoside at 39°C. It was derived from a thymidine kinase deficient, 5-bromodeoxyuridine-resistant clone (S-BUCl.42) which was originally derived from wild-type clone H-5 of FM3A cells. The temperature-sensitive mutant clone grows normally at 33°C, but not at 39°C, where it exhibits an increased frequency of multinucleate cells due to defective cytokinesis. Unlike the parental S-BUCl.42 cells, which have negligible thymidine kinase activity and are unable to incorporate3H-thymidine, the mutant incorporates substantial amounts of3H-thymidine at 33°C, although its thymidine kinase activity remains lower than that of wild-type H-5 cells. When cultures of tsCl.B59 cells are transferred to 39°C, incorporation of3H-thymidine decreases markedly. The decrease has been shown to be due to thermolability of the thymidine kinase in tsCl.B59 cells.
AB - We have isolated a mutant clone from mouse FM3A cells with temperature-sensitive defects both in cytokinesis and in thymidine kinase enzyme activity. The clone, designated tsCl.B59, was isolated after mutagenesis at 33°C followed by exposure to cytosine arabinoside at 39°C. It was derived from a thymidine kinase deficient, 5-bromodeoxyuridine-resistant clone (S-BUCl.42) which was originally derived from wild-type clone H-5 of FM3A cells. The temperature-sensitive mutant clone grows normally at 33°C, but not at 39°C, where it exhibits an increased frequency of multinucleate cells due to defective cytokinesis. Unlike the parental S-BUCl.42 cells, which have negligible thymidine kinase activity and are unable to incorporate3H-thymidine, the mutant incorporates substantial amounts of3H-thymidine at 33°C, although its thymidine kinase activity remains lower than that of wild-type H-5 cells. When cultures of tsCl.B59 cells are transferred to 39°C, incorporation of3H-thymidine decreases markedly. The decrease has been shown to be due to thermolability of the thymidine kinase in tsCl.B59 cells.
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U2 - 10.1007/BF01538982
DO - 10.1007/BF01538982
M3 - Article
C2 - 208186
AN - SCOPUS:0017877574
VL - 4
SP - 169
EP - 178
JO - Somatic Cell and Molecular Genetics
JF - Somatic Cell and Molecular Genetics
SN - 0740-7750
IS - 2
ER -