A mammalian cell mutant with temperature-sensitive thymidine kinase

Michiko Nakano, Toyozo Sekiguchi, Masa atsu Yamada

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have isolated a mutant clone from mouse FM3A cells with temperature-sensitive defects both in cytokinesis and in thymidine kinase enzyme activity. The clone, designated tsCl.B59, was isolated after mutagenesis at 33°C followed by exposure to cytosine arabinoside at 39°C. It was derived from a thymidine kinase deficient, 5-bromodeoxyuridine-resistant clone (S-BUCl.42) which was originally derived from wild-type clone H-5 of FM3A cells. The temperature-sensitive mutant clone grows normally at 33°C, but not at 39°C, where it exhibits an increased frequency of multinucleate cells due to defective cytokinesis. Unlike the parental S-BUCl.42 cells, which have negligible thymidine kinase activity and are unable to incorporate3H-thymidine, the mutant incorporates substantial amounts of3H-thymidine at 33°C, although its thymidine kinase activity remains lower than that of wild-type H-5 cells. When cultures of tsCl.B59 cells are transferred to 39°C, incorporation of3H-thymidine decreases markedly. The decrease has been shown to be due to thermolability of the thymidine kinase in tsCl.B59 cells.

Original languageEnglish (US)
Pages (from-to)169-178
Number of pages10
JournalSomatic Cell Genetics
Volume4
Issue number2
DOIs
StatePublished - Mar 1978
Externally publishedYes

Fingerprint

Thymidine Kinase
Temperature
Clone Cells
Thymidine
Cytokinesis
Cytarabine
Bromodeoxyuridine
Mutagenesis
Enzymes

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

A mammalian cell mutant with temperature-sensitive thymidine kinase. / Nakano, Michiko; Sekiguchi, Toyozo; Yamada, Masa atsu.

In: Somatic Cell Genetics, Vol. 4, No. 2, 03.1978, p. 169-178.

Research output: Contribution to journalArticle

Nakano, Michiko ; Sekiguchi, Toyozo ; Yamada, Masa atsu. / A mammalian cell mutant with temperature-sensitive thymidine kinase. In: Somatic Cell Genetics. 1978 ; Vol. 4, No. 2. pp. 169-178.
@article{6415e96582874045908eb896cf8867bc,
title = "A mammalian cell mutant with temperature-sensitive thymidine kinase",
abstract = "We have isolated a mutant clone from mouse FM3A cells with temperature-sensitive defects both in cytokinesis and in thymidine kinase enzyme activity. The clone, designated tsCl.B59, was isolated after mutagenesis at 33°C followed by exposure to cytosine arabinoside at 39°C. It was derived from a thymidine kinase deficient, 5-bromodeoxyuridine-resistant clone (S-BUCl.42) which was originally derived from wild-type clone H-5 of FM3A cells. The temperature-sensitive mutant clone grows normally at 33°C, but not at 39°C, where it exhibits an increased frequency of multinucleate cells due to defective cytokinesis. Unlike the parental S-BUCl.42 cells, which have negligible thymidine kinase activity and are unable to incorporate3H-thymidine, the mutant incorporates substantial amounts of3H-thymidine at 33°C, although its thymidine kinase activity remains lower than that of wild-type H-5 cells. When cultures of tsCl.B59 cells are transferred to 39°C, incorporation of3H-thymidine decreases markedly. The decrease has been shown to be due to thermolability of the thymidine kinase in tsCl.B59 cells.",
author = "Michiko Nakano and Toyozo Sekiguchi and Yamada, {Masa atsu}",
year = "1978",
month = "3",
doi = "10.1007/BF01538982",
language = "English (US)",
volume = "4",
pages = "169--178",
journal = "Somatic Cell and Molecular Genetics",
issn = "0740-7750",
publisher = "Springer GmbH & Co, Auslieferungs-Gesellschaf",
number = "2",

}

TY - JOUR

T1 - A mammalian cell mutant with temperature-sensitive thymidine kinase

AU - Nakano, Michiko

AU - Sekiguchi, Toyozo

AU - Yamada, Masa atsu

PY - 1978/3

Y1 - 1978/3

N2 - We have isolated a mutant clone from mouse FM3A cells with temperature-sensitive defects both in cytokinesis and in thymidine kinase enzyme activity. The clone, designated tsCl.B59, was isolated after mutagenesis at 33°C followed by exposure to cytosine arabinoside at 39°C. It was derived from a thymidine kinase deficient, 5-bromodeoxyuridine-resistant clone (S-BUCl.42) which was originally derived from wild-type clone H-5 of FM3A cells. The temperature-sensitive mutant clone grows normally at 33°C, but not at 39°C, where it exhibits an increased frequency of multinucleate cells due to defective cytokinesis. Unlike the parental S-BUCl.42 cells, which have negligible thymidine kinase activity and are unable to incorporate3H-thymidine, the mutant incorporates substantial amounts of3H-thymidine at 33°C, although its thymidine kinase activity remains lower than that of wild-type H-5 cells. When cultures of tsCl.B59 cells are transferred to 39°C, incorporation of3H-thymidine decreases markedly. The decrease has been shown to be due to thermolability of the thymidine kinase in tsCl.B59 cells.

AB - We have isolated a mutant clone from mouse FM3A cells with temperature-sensitive defects both in cytokinesis and in thymidine kinase enzyme activity. The clone, designated tsCl.B59, was isolated after mutagenesis at 33°C followed by exposure to cytosine arabinoside at 39°C. It was derived from a thymidine kinase deficient, 5-bromodeoxyuridine-resistant clone (S-BUCl.42) which was originally derived from wild-type clone H-5 of FM3A cells. The temperature-sensitive mutant clone grows normally at 33°C, but not at 39°C, where it exhibits an increased frequency of multinucleate cells due to defective cytokinesis. Unlike the parental S-BUCl.42 cells, which have negligible thymidine kinase activity and are unable to incorporate3H-thymidine, the mutant incorporates substantial amounts of3H-thymidine at 33°C, although its thymidine kinase activity remains lower than that of wild-type H-5 cells. When cultures of tsCl.B59 cells are transferred to 39°C, incorporation of3H-thymidine decreases markedly. The decrease has been shown to be due to thermolability of the thymidine kinase in tsCl.B59 cells.

UR - http://www.scopus.com/inward/record.url?scp=0017877574&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017877574&partnerID=8YFLogxK

U2 - 10.1007/BF01538982

DO - 10.1007/BF01538982

M3 - Article

C2 - 208186

AN - SCOPUS:0017877574

VL - 4

SP - 169

EP - 178

JO - Somatic Cell and Molecular Genetics

JF - Somatic Cell and Molecular Genetics

SN - 0740-7750

IS - 2

ER -