A large-volume microfiltration system for isolating suspended particulate organic matter: fabrication and assessment versus GFF filters in central North Pacific

Leslie A. Roland, Matthew D. McCarthy, Tawnya Peterson, Brett D. Walker

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We describe the construction and testing of a home-built ultrafiltration (UF) system, based on commercially available hollow fiber polysulfone membranes, for isolation of suspended particulate organic matter (POM susp) from large volumes (2000-10,000 L) of ocean water. The overall apparatus consists of two sequential UF steps: a main filtration system (100 L reservoir) driven by a stainless steel centrifugal pump, and a subsequent reduction/diafiltration system (2 L reservoir) driven by a peristaltic pump. The system can be readily assembled using off-the-shelf parts at a fraction of the cost of commercial UF systems. Our system functioned comparably to previously described commercial units. We conducted a series of tests using both surface (21 m) and mesopelagic (674 m) N. Pacific central seawater from ocean pipeline sources at the Natural Energy Laboratory Authority of Hawaii (NELHA), while simultaneously collecting GFF-POM samples. We evaluated flow rates, fouling behavior, carbon and nitrogen recoveries and compositions, and also bacteria and virus retention of ultrafiltered-POM (UPOM) using both 0.1 μm and 500 kilodalton pore size membranes. We also compared composition of UPOM versus GFF-POM, finding clear differences, which also varied between surface and mesopelagic waters. Finally, to evaluate the appeal of large-volume nitrations at NELHA to study central N. Pacific Gyre POM susp we compared our data with offshore station ALOHA.

Original languageEnglish (US)
Pages (from-to)64-80
Number of pages17
JournalLimnology and Oceanography: Methods
Volume7
Issue numberJAN.
StatePublished - Jan 2009

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Microfiltration
Ultrafiltration
Biological materials
Fabrication
Membranes
Nitration
Polysulfones
Centrifugal pumps
Fouling
Chemical analysis
Viruses
Seawater
Pore size
Water
Bacteria
Stainless steel
Pipelines
Flow rate
Pumps
Nitrogen

ASJC Scopus subject areas

  • Ocean Engineering

Cite this

A large-volume microfiltration system for isolating suspended particulate organic matter : fabrication and assessment versus GFF filters in central North Pacific. / Roland, Leslie A.; McCarthy, Matthew D.; Peterson, Tawnya; Walker, Brett D.

In: Limnology and Oceanography: Methods, Vol. 7, No. JAN., 01.2009, p. 64-80.

Research output: Contribution to journalArticle

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