A human trophoblastic isozyme (lactate dehydrogenase-z) associated with choriocarcinoma

Michael J. Siciliano, Mary E. Bordelon-Riser, Ralph S. Freedman, Peter O. Kohler

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Abstract

A unique electrophoretic form of lactate dehydrogenase (LDH-Z), formerly observed in a choriocarcinoma cell line (JEG-3) and first-trimester placenta, has been shown to be the same as that produced in hydatidiform mole and term placenta. We have also observed LDH-Z in second-trimester placenta, choriocarcinoma metastasized to the liver, and five of five additional independently derived (from different patients) choriocarcinoma cell lines. The only exception to the production of LDH-Z in a choriocarcinoma was in the cell line BEWO, which was established from the same tumor as JEG-3. Since BEWO has been in culture for over 200 passages more than any of the independently derived lines, its lack of LDH-Z is viewed as being consistent with the loss of certain expressional characteristics upon such long-term culture. Analysis of JEG-3 sub-clones revealed the expression of LDH-Z to be independent of the products of the LDH-A genetic locus. Present data do not allow us to determine whether LDH-Z is the product of a newly discovered LDH locus or is a modified form of the product of the LDH-B locus. LDH-Z has not been observed in other human tissues, nor have we observed it in homogenates prepared from over 60 cell lines established from a wide variety of human neoplasms. We therefore conclude that LDH-Z is an isozyme associated with human choriocarcinoma and is indicative of the trophoblastic origin of the cells. 3.

Original languageEnglish (US)
Pages (from-to)283-287
Number of pages5
JournalCancer Research
Volume40
Issue number2
StatePublished - Feb 1 1980

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Siciliano, M. J., Bordelon-Riser, M. E., Freedman, R. S., & Kohler, P. O. (1980). A human trophoblastic isozyme (lactate dehydrogenase-z) associated with choriocarcinoma. Cancer Research, 40(2), 283-287.