A human trophoblastic isozyme (lactate dehydrogenase-Z) associated with choriocarcinoma

M. J. Siciliano, M. E. Bordelon-Riser, R. S. Freedman, Peter Kohler

Research output: Contribution to journalArticle

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Abstract

A unique electrophoretic form of lactate dehydrogenase (LDH-Z) formerly observed in a choriocarcinoma cell line (JEG-3) and first-trimester placenta, has been shown to be the same as that produced in hydatidiform mole and term placenta. We have also observed LDH-Z in second trimester placenta, choriocarcinoma metastasized to the liver, and five of five additional independently derived (from different patients) choriocarcinoma cell lines. The only exception to the production of LDH-Z in a choriocarcinoma was in the cell line BEWO, which was established from the same tumor as JEG-3. Since BEWO has been in culture for over 200 passages more than any of the independently derived lines, its lack of LDH-Z is viewed as being consistent with the loss of certain expressional characteristics upon such long-term culture. Analysis of JEG-3 subclones revealed the expression of LDH-Z to be independent of the products of the LDH-A genetic locus. Present data do not allow us to determine whether LDH-Z is the product of a newly discovered LDH locus or is a modified form of the product of the LDH-B locus. LDH-Z has not been observed in other human tissues, nor have we observed it in homogenates prepared from over 60 cell lines established from a wide variety of human neoplasms. We therefore conclude that LDH-Z is an isozyme associated with human choriocarcinoma and is indicative of the trophoblastic origin of the cells.

Original languageEnglish (US)
Pages (from-to)283-287
Number of pages5
JournalCancer Research
Volume40
Issue number2
StatePublished - 1980
Externally publishedYes

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Choriocarcinoma
L-Lactate Dehydrogenase
Isoenzymes
Placenta
Cell Line
Hydatidiform Mole
Genetic Loci
Second Pregnancy Trimester
First Pregnancy Trimester
Neoplasms
Liver

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Siciliano, M. J., Bordelon-Riser, M. E., Freedman, R. S., & Kohler, P. (1980). A human trophoblastic isozyme (lactate dehydrogenase-Z) associated with choriocarcinoma. Cancer Research, 40(2), 283-287.

A human trophoblastic isozyme (lactate dehydrogenase-Z) associated with choriocarcinoma. / Siciliano, M. J.; Bordelon-Riser, M. E.; Freedman, R. S.; Kohler, Peter.

In: Cancer Research, Vol. 40, No. 2, 1980, p. 283-287.

Research output: Contribution to journalArticle

Siciliano, MJ, Bordelon-Riser, ME, Freedman, RS & Kohler, P 1980, 'A human trophoblastic isozyme (lactate dehydrogenase-Z) associated with choriocarcinoma', Cancer Research, vol. 40, no. 2, pp. 283-287.
Siciliano, M. J. ; Bordelon-Riser, M. E. ; Freedman, R. S. ; Kohler, Peter. / A human trophoblastic isozyme (lactate dehydrogenase-Z) associated with choriocarcinoma. In: Cancer Research. 1980 ; Vol. 40, No. 2. pp. 283-287.
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