A human lymphocyte homing receptor, the Hermes antigen, is related to cartilage proteoglycan core and link proteins

Leslie A. Goldstein, David F H Zhou, Louis Picker, Catherine N. Minty, Robert F. Bargatze, Jie F. Ding, Eugene C. Butcher

Research output: Contribution to journalArticle

376 Citations (Scopus)

Abstract

Lymphocyte interactions with high endothelial venules (HEV) during extravasation into lymphoid tissues involve an 85-95 kd class of lymphocyte surface glycoprotein(s), gp90Hermes (CD44). We report here the cloning of cDNA for gp90Hermes expressed in a mucosal HEV-binding B lymphoblastoid cell line, KCA. Northern hybridization revealed the presence of three invariant RNA bands at 1.5, 2.2, and 4.5 kb in mucosal HEV-, lymph node HEV-, or dual-binding cells. The deduced amino acid sequence predicts a mature protein with a C-terminal cytoplasmic tail, a hydrophobic transmembrane domain of 23 amino acids, and an N-terminal extracellular region of 248 amino acids. A proximal extracellular domain is the probable region of O-glycosylation and chondroitin sulfate linkage and displays at least two of the three immunodominant epitope clusters of native gp90Hermes. A distal region contains the majority of potential N-glycosylation sites and cysteines, and exhibits a striking homology to tandemly repeated domains of the cartilage link and proteoglycan core proteins. No significant similarities were found to the immunoglobulin, integrin, or cadherin gene families. Thus gp90Hermes represents a novel class of integral membrane protein involved in lymphocyte-endothelial cell interactions and lymphocyte homing.

Original languageEnglish (US)
Pages (from-to)1063-1072
Number of pages10
JournalCell
Volume56
Issue number6
DOIs
StatePublished - Mar 24 1989
Externally publishedYes

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Lymphocyte Homing Receptors
Venules
Lymphocytes
Cartilage
Proteoglycans
Glycosylation
Antigens
Amino Acids
Immunodominant Epitopes
Chondroitin Sulfates
Cloning
Membrane Glycoproteins
Endothelial cells
Lymphoid Tissue
Cadherins
Integrins
Cell Communication
Cysteine
Tail
Immunoglobulins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Goldstein, L. A., Zhou, D. F. H., Picker, L., Minty, C. N., Bargatze, R. F., Ding, J. F., & Butcher, E. C. (1989). A human lymphocyte homing receptor, the Hermes antigen, is related to cartilage proteoglycan core and link proteins. Cell, 56(6), 1063-1072. https://doi.org/10.1016/0092-8674(89)90639-9

A human lymphocyte homing receptor, the Hermes antigen, is related to cartilage proteoglycan core and link proteins. / Goldstein, Leslie A.; Zhou, David F H; Picker, Louis; Minty, Catherine N.; Bargatze, Robert F.; Ding, Jie F.; Butcher, Eugene C.

In: Cell, Vol. 56, No. 6, 24.03.1989, p. 1063-1072.

Research output: Contribution to journalArticle

Goldstein, LA, Zhou, DFH, Picker, L, Minty, CN, Bargatze, RF, Ding, JF & Butcher, EC 1989, 'A human lymphocyte homing receptor, the Hermes antigen, is related to cartilage proteoglycan core and link proteins', Cell, vol. 56, no. 6, pp. 1063-1072. https://doi.org/10.1016/0092-8674(89)90639-9
Goldstein, Leslie A. ; Zhou, David F H ; Picker, Louis ; Minty, Catherine N. ; Bargatze, Robert F. ; Ding, Jie F. ; Butcher, Eugene C. / A human lymphocyte homing receptor, the Hermes antigen, is related to cartilage proteoglycan core and link proteins. In: Cell. 1989 ; Vol. 56, No. 6. pp. 1063-1072.
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